CSF2 Impairs Nrf2 Signaling through the Akt/Mtor Pathway in the Development of Bladder Cancer

Yu, Xi; Li, Shenglan; Ke, Shuai; Ye, Chenglin; Wang, Qinghua; Wang, Huaxin; Wang, Lei

doi:10.7150/jca.94343

International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

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J Cancer 2024; 15(11):3242-3253. doi:10.7150/jca.94343 This issue Cite

Research Paper

CSF2 Impairs Nrf2 Signaling through the Akt/Mtor Pathway in the Development of Bladder Cancer

Xi Yu^1*, Shenglan Li^2*, Shuai Ke^3*, Chenglin Ye³, Qinghua Wang³, Huaxin Wang^1✉, Lei Wang^3✉

1. Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.
2. Department of Radiography, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.
3. Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.
* These authors have contributed equally to this work.

Citation:

Yu X, Li S, Ke S, Ye C, Wang Q, Wang H, Wang L. CSF2 Impairs Nrf2 Signaling through the Akt/Mtor Pathway in the Development of Bladder Cancer. J Cancer 2024; 15(11):3242-3253. doi:10.7150/jca.94343. https://www.jcancer.org/v15p3242.htm

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Abstract

Bladder Cancer (BCa) is one of the most common cancers of the urinary system. Colony-stimulating factor 2 (CSF2) is involved in many cancers, but not BCa. We investigated the effect of CSF2 on BCa in this study and the underlying molecular mechanisms. CSF2 mRNA levels in BCa were analyzed using the Cancer Genome Atlas (TCGA) database. Western blot was conducted to verify CSF2 expression in BCa tissue samples and cell lines. The effect of CSF2 on the growth of BCa cells was assessed by CCK8 and colony formation. To determine the migration and invasion capabilities of BCa cells, transwell analysis and wound healing assays were conducted. Next, western blot was used to explore the underlying mechanism. In the end, a xenografted BCa mouse model was established to examine the effects of CSF2 on tumorigenesis in vivo. Results showed that CSF2 mRNA was upregulated in BCa samples. Knocking down CSF2 significantly inhibited the proliferation and tumorigenesis of BCa cells in vitro and in vivo. Mechanism analysis revealed that CSF2 knockdown inhibited the proliferation and invasion of BCa cells via AKT/mTOR signaling. Based on these results, CSF2 promotes the proliferation and tumorigenesis of BCa.

Keywords: bladder cancer, CSF2, AKT/mTOR pathway, Nrf2, EMT

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APA

Yu, X., Li, S., Ke, S., Ye, C., Wang, Q., Wang, H., Wang, L. (2024). CSF2 Impairs Nrf2 Signaling through the Akt/Mtor Pathway in the Development of Bladder Cancer. Journal of Cancer, 15(11), 3242-3253. https://doi.org/10.7150/jca.94343.

ACS

Yu, X.; Li, S.; Ke, S.; Ye, C.; Wang, Q.; Wang, H.; Wang, L. CSF2 Impairs Nrf2 Signaling through the Akt/Mtor Pathway in the Development of Bladder Cancer. J. Cancer 2024, 15 (11), 3242-3253. DOI: 10.7150/jca.94343.

NLM

CSE

Yu X, Li S, Ke S, Ye C, Wang Q, Wang H, Wang L. 2024. CSF2 Impairs Nrf2 Signaling through the Akt/Mtor Pathway in the Development of Bladder Cancer. J Cancer. 15(11):3242-3253.

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