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J Cancer 2023; 14(7):1088-1106. doi:10.7150/jca.83050 This issue Cite

Research Paper

In Vitro Effects of Low-energy Ultrasound Treatment on Healthy CD3/CD8+ Lymphocytes, Red blood cells, Acute Myeloid leukemia cells, and Jurkat cell line

Francesco De Leonardis1✉*, Simona Nicole Barile1*, Claudia Cianci3, Isabella Pisano1, Giuseppe Merla2, Giovanni Pappalettera3✉, Caterina Casavola3, Carmine Pappalettere3

1. Dept. Bioscience Biotechnology and Environment, University of Bari” A. Moro”, Bari, 70125, Italy.
2. Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, 8013, Italy.
3. Dept. of Mechanics, Mathematics, and Management, Polytechnic University of Bari, Bari, 70125, Italy.
*Co-first authors

✉ Corresponding authors: Francesco De Leonardis, Dept. of Biosciences Biotechnology and Environment, University of Bari “A. Moro”, 4, Orabona street (BA), 70125 Bari, Italy, E-mail: francesco.deleonardisit; Giovanni Pappalettera, De More

Citation:
De Leonardis F, Barile SN, Cianci C, Pisano I, Merla G, Pappalettera G, Casavola C, Pappalettere C. In Vitro Effects of Low-energy Ultrasound Treatment on Healthy CD3/CD8+ Lymphocytes, Red blood cells, Acute Myeloid leukemia cells, and Jurkat cell line. J Cancer 2023; 14(7):1088-1106. doi:10.7150/jca.83050. https://www.jcancer.org/v14p1088.htm
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Abstract

Graphic abstract

The study of the biological effects of low-energy ultrasound and its applications is a rapidly expanding research area. Low-energy ultrasound could be used as anti-tumoral therapy with or without the pharmacological combination even if the second situation has been scarcely investigated up to now. Very little information is available about the ultrasound effects on healthy red blood cells, CD3, and mainly CD8 subset lymphocytes which is the main subset cell having cytotoxic function towards cancer cells. In this study, we investigated in vitro the bioeffects of low energy ultrasound on red blood cells and PBMCs isolated from healthy donors as well as on two myeloid leukemia cell lines (OCI- AML-3 MOLM-13) and lymphoblastic Jurkat cell line. Using low-energy ultrasound (US), a study was conducted to determine how it affects CD3/CD8 lymphocytes and leukemia cells, as well as its potential role in treating blood cancers, by analyzing changes in mitochondrial membrane potential, phosphatidylserine asymmetry, morphological changes for myeloid AML cell lines, proliferation and cytotoxic activation of healthy lymphocytes, and apoptosis for RBCs after US exposure. Overall, we demonstrated that CD3/CD8 lymphocytes proliferation/activation and cytotoxic functions are fully preserved after ultrasound treatments, whereas leukemia cell lines undergo apoptosis and stop proliferating suggesting a potential method of treating blood cancer.

Keywords: Lymphocytes, Red blood cells, CD3/CD8 lymphocytes proliferation/activation and cytotoxic functions

Citation styles

APA
De Leonardis, F., Barile, S.N., Cianci, C., Pisano, I., Merla, G., Pappalettera, G., Casavola, C., Pappalettere, C. (2023). In Vitro Effects of Low-energy Ultrasound Treatment on Healthy CD3/CD8+ Lymphocytes, Red blood cells, Acute Myeloid leukemia cells, and Jurkat cell line. Journal of Cancer, 14(7), 1088-1106. https://doi.org/10.7150/jca.83050.

ACS
De Leonardis, F.; Barile, S.N.; Cianci, C.; Pisano, I.; Merla, G.; Pappalettera, G.; Casavola, C.; Pappalettere, C. In Vitro Effects of Low-energy Ultrasound Treatment on Healthy CD3/CD8+ Lymphocytes, Red blood cells, Acute Myeloid leukemia cells, and Jurkat cell line. J. Cancer 2023, 14 (7), 1088-1106. DOI: 10.7150/jca.83050.

NLM
De Leonardis F, Barile SN, Cianci C, Pisano I, Merla G, Pappalettera G, Casavola C, Pappalettere C. In Vitro Effects of Low-energy Ultrasound Treatment on Healthy CD3/CD8+ Lymphocytes, Red blood cells, Acute Myeloid leukemia cells, and Jurkat cell line. J Cancer 2023; 14(7):1088-1106. doi:10.7150/jca.83050. https://www.jcancer.org/v14p1088.htm

CSE
De Leonardis F, Barile SN, Cianci C, Pisano I, Merla G, Pappalettera G, Casavola C, Pappalettere C. 2023. In Vitro Effects of Low-energy Ultrasound Treatment on Healthy CD3/CD8+ Lymphocytes, Red blood cells, Acute Myeloid leukemia cells, and Jurkat cell line. J Cancer. 14(7):1088-1106.

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