J Cancer 2022; 13(4):1073-1085. doi:10.7150/jca.68403 This issue Cite

Research Paper

Histidine-rich calcium binding protein promotes gastric cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition through Raf/MEK/ERK signaling

Chao Wang1*, Chuanfu Ren2*, Qiongyuan Hu1*, Xiaofei Shen1*, Meng Wang2, Zhi Yang2, En Xu1, Xingzhou Wang1, Zijian Li2, Heng Yu2, Qingzhao Feng2, Liang Zhang2, Xuefeng Xia1, Song Liu1✉, Wenxian Guan1✉

1. Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
2. Department of General Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing 210008, China.
*These authors contributed equally to this work.

Citation:
Wang C, Ren C, Hu Q, Shen X, Wang M, Yang Z, Xu E, Wang X, Li Z, Yu H, Feng Q, Zhang L, Xia X, Liu S, Guan W. Histidine-rich calcium binding protein promotes gastric cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition through Raf/MEK/ERK signaling. J Cancer 2022; 13(4):1073-1085. doi:10.7150/jca.68403. https://www.jcancer.org/v13p1073.htm
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Abstract

Graphic abstract

Histidine-rich calcium binding protein (HRC) is a new type of Ca2+ homeostasis regulator, which acts as a nonnegligible role in regulating intracellular calcium homeostasis. Here, we demonstrated that HRC expression was upregulated in human gastric cancer (GC) samples, and its expression level was closely correlated with the overall survival (OS) rate of GC patients and the malignant potential of GC cell lines. Knockdown of HRC inhibited migration, invasion, and proliferation of GC cell lines in vitro, while HRC overexpression promoted GC cell migration, invasion, and proliferation in vitro, as well as the growth of subcutaneous tumors and peritoneal tumors in vivo. In terms of the mechanism, knockdown of HRC reduced the intracellular calcium ion level and the CaM protein level. Through cell function experiments, we found that HRC regulated the Raf/MEK/ERK pathway through Ca2+/CaM signaling and ultimately affected the epithelial‑mesenchyme transition (EMT) of GC. In summary, we revealed that HRC represents a potential target for GC treatment.

Keywords: HRC, Calcium homeostasis, EMT, Gastric cancer, Metastasis


Citation styles

APA
Wang, C., Ren, C., Hu, Q., Shen, X., Wang, M., Yang, Z., Xu, E., Wang, X., Li, Z., Yu, H., Feng, Q., Zhang, L., Xia, X., Liu, S., Guan, W. (2022). Histidine-rich calcium binding protein promotes gastric cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition through Raf/MEK/ERK signaling. Journal of Cancer, 13(4), 1073-1085. https://doi.org/10.7150/jca.68403.

ACS
Wang, C.; Ren, C.; Hu, Q.; Shen, X.; Wang, M.; Yang, Z.; Xu, E.; Wang, X.; Li, Z.; Yu, H.; Feng, Q.; Zhang, L.; Xia, X.; Liu, S.; Guan, W. Histidine-rich calcium binding protein promotes gastric cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition through Raf/MEK/ERK signaling. J. Cancer 2022, 13 (4), 1073-1085. DOI: 10.7150/jca.68403.

NLM
Wang C, Ren C, Hu Q, Shen X, Wang M, Yang Z, Xu E, Wang X, Li Z, Yu H, Feng Q, Zhang L, Xia X, Liu S, Guan W. Histidine-rich calcium binding protein promotes gastric cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition through Raf/MEK/ERK signaling. J Cancer 2022; 13(4):1073-1085. doi:10.7150/jca.68403. https://www.jcancer.org/v13p1073.htm

CSE
Wang C, Ren C, Hu Q, Shen X, Wang M, Yang Z, Xu E, Wang X, Li Z, Yu H, Feng Q, Zhang L, Xia X, Liu S, Guan W. 2022. Histidine-rich calcium binding protein promotes gastric cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition through Raf/MEK/ERK signaling. J Cancer. 13(4):1073-1085.

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