J Cancer 2022; 13(2):565-578. doi:10.7150/jca.62033 This issue
1. Department of General Surgery, the First Affiliated Hospital of Nanchang University, Nanchang, China.
2. Laboratory of Digestive Surgery, Nanchang University, Nanchang, China.
3. Queen Mary School, Medical Department of Nanchang University, Nanchang, China.
4. Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang, China.
*These authors contributed equally to this work.
RNF114 (E3 ubiquitin ligase RING finger protein 114) was first identified as a zinc-binding protein that promotes psoriasis development; however, its role in gastric cancer is still unclear. We explored the relationship between RNF114 and gastric cancer using bioinformatics and molecular biology techniques. The results showed that RNF114 was highly expressed in gastric cancer and negatively correlated with the patient's prognosis. Functional assays suggested that RNF114 silencing suppressed the proliferation and metastasis of gastric cancer cells to a certain extent. Further studies showed that RNF114 expression was potentially targeted by miR-218-5p and methylation modification, and mediated downstream EGR1 (early growth response 1) degradation by the ubiquitylation approach. Together, the present results highlight the detrimental effects of RNF114 overexpression in gastric cancer and contribute to a better understanding of the mechanisms underlying RNF114 functionality.
Keywords: RNF114, EGR1, miR-218-5p, methylation, ubiquitylation, gastric cancer