Department of Interventional Radiology, The First Affiliated Hospital of Nanjing Medical University; Gulou, Nanjing 210029, P.R. China. *These authors contributed equally to this work.
✉ Corresponding authors: Dr Hai-Bin Shi and Dr Wei-Zhong Zhou, Department of Interventional Radiology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Gulou, Nanjing 210029, P.R. China. E-mail: shihbsina.com; E-mail: xmjbq007com.More
Citation:
Zhang JX, Yang W, Wu JZ, Zhou C, Liu S, Shi HB, Zhou WZ. MicroRNA-32-5p inhibits epithelial-mesenchymal transition and metastasis in lung adenocarcinoma by targeting SMAD family 3. J Cancer 2021; 12(8):2258-2267. doi:10.7150/jca.48387. https://www.jcancer.org/v12p2258.htm
Non-small cell lung cancer (NSCLC) is the leading cause of cancer-associated death worldwide. MicroRNA (miRNA)-32-5p is as an important cancer-associated miRNA in different types cancer. To date, the role of miR-32-5p in the migration and invasion of NSCLC remains unknown. In the present study, a Transwell assay was performed to investigate the role of miR-32-5p in lung adenocarcinoma. miR-32-5p expression level was determined via reverse transcription-quantitative PCR in 24 pairs of NSCLC and adjacent normal tissues. SMAD family member 3 (SMAD3) was considered as a novel target gene by luciferase reporter assay and western blot in NSCLC. The present study demonstrated that miR-32-5p is frequently downregulated in NSCLC tissues. The overexpression of miR-32-5p resulted in the inhibition of migratory and invasive abilities in NSCLC cells. Thus, SMAD3 was identified as a target of miR-32-5p, and its expression was negatively correlated with miR-32-5p expression in clinical NSCLC tissues. Overall, these findings indicate that miR-32-5p serves as a tumor suppressor by targeting SMAD3. Thus, miR-32-5p may be a potential therapeutic target for the treatment of lung adenocarcinoma.
Zhang, J.X., Yang, W., Wu, J.Z., Zhou, C., Liu, S., Shi, H.B., Zhou, W.Z. (2021). MicroRNA-32-5p inhibits epithelial-mesenchymal transition and metastasis in lung adenocarcinoma by targeting SMAD family 3. Journal of Cancer, 12(8), 2258-2267. https://doi.org/10.7150/jca.48387.
ACS
Zhang, J.X.; Yang, W.; Wu, J.Z.; Zhou, C.; Liu, S.; Shi, H.B.; Zhou, W.Z. MicroRNA-32-5p inhibits epithelial-mesenchymal transition and metastasis in lung adenocarcinoma by targeting SMAD family 3. J. Cancer 2021, 12 (8), 2258-2267. DOI: 10.7150/jca.48387.
NLM
Zhang JX, Yang W, Wu JZ, Zhou C, Liu S, Shi HB, Zhou WZ. MicroRNA-32-5p inhibits epithelial-mesenchymal transition and metastasis in lung adenocarcinoma by targeting SMAD family 3. J Cancer 2021; 12(8):2258-2267. doi:10.7150/jca.48387. https://www.jcancer.org/v12p2258.htm
CSE
Zhang JX, Yang W, Wu JZ, Zhou C, Liu S, Shi HB, Zhou WZ. 2021. MicroRNA-32-5p inhibits epithelial-mesenchymal transition and metastasis in lung adenocarcinoma by targeting SMAD family 3. J Cancer. 12(8):2258-2267.
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