J Cancer 2021; 12(2):467-473. doi:10.7150/jca.49641

Research Paper

MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway

Tonglei Chen1#, Zhenshi Chen2,3#, Xiaoning Lian4#, Weidong Wu2,3, Lei Chu2,3, Shaoru Zhang2,3✉, Lihui Wang2,3✉

1. Suzhou Ninth People's Hospital, Suzhou, Jiangsu 215200, China.
2. Danyang People's Hospital of Jiangsu Province, Danyang, Jiangsu 212300, China.
3. Danyang Hospital Affiliated to Nantong University, Danyang, Jiangsu 212300, China.
4. Department of Assay Development, TOT BIOPHARM co., LTD, Suzhou, Jiangsu 215024, China.
#These authors contributed equally to this work.

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Citation:
Chen T, Chen Z, Lian X, Wu W, Chu L, Zhang S, Wang L. MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway. J Cancer 2021; 12(2):467-473. doi:10.7150/jca.49641. Available from https://www.jcancer.org/v12p0467.htm

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Abstract

Objective: To investigate the high expression of MUC15 in promoting proliferation, migration and invasion in osteosarcoma (OS) cell and its potential mechanism.

Methods: The expressions of MUC15 in OS patients were analyzed from GEO Datasets, tumor cell lines and clinical samples. The roles of MUC15 in OS were explored by CCK-8, flow cytometry, transwell and western blot assay, respectively.

Results: MUC15 was highly expressed in osteosarcoma, and there was a significant negative correlation between MUC15 and the prognosis. Knockdown of MUC15 in HOS and U-2OS could promote tumor cell apoptosis, down-regulate the expression of MMP2/9, reduce the epithelial interstitial transition and silence the Wnt/b-Catenin signal pathway.

Conclusion: The high-expression of MUC15 promotes the proliferation, migration and invasion of osteosarcoma through anti-apoptosis, increasing the invasive ability by epithelial interstitial transition, and activating the Wnt/b-Catenin signal pathway.

Keywords: Osteosarcoma, MUC 15, proliferation, migration, invasion