Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis

Chen, Zhixiang; Liang, Qianxin; Zeng, Hua; Zhao, Qing; Guo, Zhaodi; Zhong, Rihui; Xie, Manlin; Cai, Xiuping; Su, Jing; He, Zhiliang; Zheng, Lei; Zhao, Kewei

doi:10.7150/jca.48531

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International Journal of Medical Sciences

Theranostics

Journal of Genomics

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J Cancer 2020; 11(21):6445-6453. doi:10.7150/jca.48531 This issue Cite

Research Paper

Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis

Zhixiang Chen^2#, Qianxin Liang^1#, Hua Zeng³, Qing Zhao², Zhaodi Guo², Rihui Zhong³, Manlin Xie¹, Xiuping Cai², Jing Su², Zhiliang He², Lei Zheng^1,2✉, Kewei Zhao^1,2✉

1. Guangzhou University of Chinese Medicine, Guangdong Guangzhou, 510006, China.
2. The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Guangzhou, 510378, China.
3. Department of Clinical Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 510120, China.
#Co-first authors.

Citation:

Chen Z, Liang Q, Zeng H, Zhao Q, Guo Z, Zhong R, Xie M, Cai X, Su J, He Z, Zheng L, Zhao K. Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis. J Cancer 2020; 11(21):6445-6453. doi:10.7150/jca.48531. https://www.jcancer.org/v11p6445.htm

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Abstract

The main diagnostic indicators of ovarian cancer (OC), including carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4), show good sensitivity and poor specificity or vice versa. This study investigated changes in CA125 and HE4 expression and their correlation in serum-derived exosomes of 55 patients with OC (OC group), 33 patients with malignant tumors (non-OC group), and 55 normal controls (NC group). We compared serum and exosomal CA125 and HE4 levels to determine whether their contents in exosomes were elevated. We also compared the diagnostic efficacy of serum HE4, serum CA125, exosomal CA125, and serum HE4+exosomal CA125 in OC using the receiver operating characteristic (ROC) curve. CA125 levels in serum-derived exosomes in all groups significantly increased (P < 0.0001) compared with serum CA125 levels. HE4 was undetected in exosomes. The ROC curve showed the following values: serum CA125: 0.9093 (area), 87.27% (sensitivity), and 90.91% (specificity); serum HE4: 0.9302, 83.64%, and 94.55%; exosomal CA125: 0.9755, 94.55%, and 92.73%; and serum HE4+exosomal CA125: 0.9861, 96.36%, and 92.73%. In conclusion, CA125 can be detected at higher levels in exosomes than in serum, significantly improving OC diagnosis sensitivity. The serum HE4+exosomal CA125 combination significantly improves OC diagnostic efficiency.

Keywords: ovarian cancer, exosome, carbohydrate antigen 125, human epididymis protein 4

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APA

Chen, Z., Liang, Q., Zeng, H., Zhao, Q., Guo, Z., Zhong, R., Xie, M., Cai, X., Su, J., He, Z., Zheng, L., Zhao, K. (2020). Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis. Journal of Cancer, 11(21), 6445-6453. https://doi.org/10.7150/jca.48531.

ACS

Chen, Z.; Liang, Q.; Zeng, H.; Zhao, Q.; Guo, Z.; Zhong, R.; Xie, M.; Cai, X.; Su, J.; He, Z.; Zheng, L.; Zhao, K. Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis. J. Cancer 2020, 11 (21), 6445-6453. DOI: 10.7150/jca.48531.

NLM

CSE

Chen Z, Liang Q, Zeng H, Zhao Q, Guo Z, Zhong R, Xie M, Cai X, Su J, He Z, Zheng L, Zhao K. 2020. Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis. J Cancer. 11(21):6445-6453.

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