J Cancer 2020; 11(12):3424-3432. doi:10.7150/jca.40233

Research Paper

MiR-425 Promotes Migration and Invasion in Bladder Cancer by Targeting Dickkopf 3

Jin-zhuo Ning*, Wei-min Yu*, Fan Cheng, Ting Rao, Yuan Ruan

Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, P.R.China
*Contributed equally

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Citation:
Ning Jz, Yu Wm, Cheng F, Rao T, Ruan Y. MiR-425 Promotes Migration and Invasion in Bladder Cancer by Targeting Dickkopf 3. J Cancer 2020; 11(12):3424-3432. doi:10.7150/jca.40233. Available from http://www.jcancer.org/v11p3424.htm

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Abstract

Background: Bladder cancer (BC) is a common malignancy with high morbidity and mortality. MicroRNAs (miRNAs) are critical post-transcriptional regulators in various cancers. This study aimed to investigate the effect of miR-425 on the migration and invasion of BC.

Methods: The expression of miR-425 and Dickkopf 3 (DKK3) was examined in clinical BC specimens. T24 and 5637 BC cell lines were employed and transfected with miR-425 inhibitors. The correlation between miR-425 and DKK3 was determined by a luciferase reporter assay. Cell migration and invasion capacity were measured by wound healing and Transwell assays. The expression levels of DKK3, E-cadherin, N-cadherin and vimentin were analysed by Western blotting and qRT-PCR.

Results: miR-425 was negatively correlated with the expression of DKK3 in clinical BC specimens. Further studies identified DKK-3 as a direct target of miR-425. Moreover, knockdown of miR-425 promoted the expression of DKK3 and suppressed cell migration and invasion capacity. miR-425 silencing increased E-cadherin levels but decreased vimentin and N-cadherin protein levels in T24 and 5637 cells.

Conclusion: Our study indicated that miR-425 promoted the migration and invasion of BC via targeting DKK3.

Keywords: miR-425, DKK-3, bladder cancer, migration, invasion