J Cancer 2020; 11(9):2476-2484. doi:10.7150/jca.37748 This issue Cite

Research Paper

MicroRNA-19a-3p regulates cell growth through modulation of the PIK3IP1-AKT pathway in hepatocellular carcinoma

Hai-Xiang Sun1,*, Zhang-Fu Yang2,*, Wei-Guo Tang3,*, Ai-Wu Ke1, Wei-ren Liu2, Yan Li1, Chao Gao4, Bo Hu2, Pei-Yao Fu2, Min-Cheng Yu2, Bo-Wen Gao2, Ying-hong Shi2, Jia Fan1,2,4,✉, Yang Xu2,✉

1. Liver Cancer Institute, Zhongshan Hospital, Fudan University; Key Laboratory of Carcinogenesis and Cancer Invasion (Fudan University), Ministry of Education, Shanghai 200032, China
2. Department of Liver Surgery and Transplantation, Zhongshan Hospital, Fudan University; Key Laboratory of Carcinogenesis and Cancer Invasion (Fudan University), Ministry of Education, Shanghai 200032, China
3. Minhang Hospital, Fudan University; Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 200032, China
4. Institute of Biomedical Sciences, Fudan University, Shanghai 200032, China
* contributed equally

Citation:
Sun HX, Yang ZF, Tang WG, Ke AW, Liu Wr, Li Y, Gao C, Hu B, Fu PY, Yu MC, Gao BW, Shi Yh, Fan J, Xu Y. MicroRNA-19a-3p regulates cell growth through modulation of the PIK3IP1-AKT pathway in hepatocellular carcinoma. J Cancer 2020; 11(9):2476-2484. doi:10.7150/jca.37748. https://www.jcancer.org/v11p2476.htm
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Abstract

There are some controversies about the involvement of microRNA (miR)-19a-3p in hepatocellular carcinoma (HCC) biology, even though many studies have shown that it plays an important role in cancer. In this study, we found that miR-19a-3p is usually overexpressed in HCC tissues compared with corresponding peritumorous tissues, and its expression was associated with tumor size and poor overall survival. MiR-19a-3p promoted cell proliferation significantly, and more cells were found in the S phase. In vivo, miR-19a-3p promoted liver tumor growth, and more HCC cells were found in the active cell cycle. Sequencing and bioinformatics analysis predicted that PIK3IP1 is a likely target gene of miR-19a-3p, and we next confirmed it by luciferase and rescue assays. Altogether, our data showed an important role of PIK3IP1 downregulation by miR-19a-3p in HCC progression, and the miR-19a-3p-PIK3IP1-AKT pathway may be a potential therapeutic target.

Keywords: microRNA-19a-3p, miR-19a-3p, phosphatidyl inositol 3-kinase interacting protein 1, PIK3IP1, hepatocellular carcinoma, cell growth


Citation styles

APA
Sun, H.X., Yang, Z.F., Tang, W.G., Ke, A.W., Liu, W.r., Li, Y., Gao, C., Hu, B., Fu, P.Y., Yu, M.C., Gao, B.W., Shi, Y.h., Fan, J., Xu, Y. (2020). MicroRNA-19a-3p regulates cell growth through modulation of the PIK3IP1-AKT pathway in hepatocellular carcinoma. Journal of Cancer, 11(9), 2476-2484. https://doi.org/10.7150/jca.37748.

ACS
Sun, H.X.; Yang, Z.F.; Tang, W.G.; Ke, A.W.; Liu, W.r.; Li, Y.; Gao, C.; Hu, B.; Fu, P.Y.; Yu, M.C.; Gao, B.W.; Shi, Y.h.; Fan, J.; Xu, Y. MicroRNA-19a-3p regulates cell growth through modulation of the PIK3IP1-AKT pathway in hepatocellular carcinoma. J. Cancer 2020, 11 (9), 2476-2484. DOI: 10.7150/jca.37748.

NLM
Sun HX, Yang ZF, Tang WG, Ke AW, Liu Wr, Li Y, Gao C, Hu B, Fu PY, Yu MC, Gao BW, Shi Yh, Fan J, Xu Y. MicroRNA-19a-3p regulates cell growth through modulation of the PIK3IP1-AKT pathway in hepatocellular carcinoma. J Cancer 2020; 11(9):2476-2484. doi:10.7150/jca.37748. https://www.jcancer.org/v11p2476.htm

CSE
Sun HX, Yang ZF, Tang WG, Ke AW, Liu Wr, Li Y, Gao C, Hu B, Fu PY, Yu MC, Gao BW, Shi Yh, Fan J, Xu Y. 2020. MicroRNA-19a-3p regulates cell growth through modulation of the PIK3IP1-AKT pathway in hepatocellular carcinoma. J Cancer. 11(9):2476-2484.

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