J Cancer 2019; 10(21):5306-5314. doi:10.7150/jca.34806 This issue Cite

Research Paper

5-Hydroxymethylcytosine and ten-eleven translocation dioxygenases in head and neck carcinoma

Kiyoshi Misawa1✉, Satoshi Yamada1, Masato Mima1, Takuya Nakagawa2, Tomoya Kurokawa2, Atsushi Imai1, Daiki Mochizuki1, Kotaro Morita1, Ryuji Ishikawa1, Shiori Endo1, Yuki Misawa1

1. Department of Otolaryngology/Head and Neck Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan
2. Department of Otorhinolaryngology/Head and Neck Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan

Citation:
Misawa K, Yamada S, Mima M, Nakagawa T, Kurokawa T, Imai A, Mochizuki D, Morita K, Ishikawa R, Endo S, Misawa Y. 5-Hydroxymethylcytosine and ten-eleven translocation dioxygenases in head and neck carcinoma. J Cancer 2019; 10(21):5306-5314. doi:10.7150/jca.34806. https://www.jcancer.org/v10p5306.htm
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Abstract

Ten-eleven translocation (TET) enzymes are implicated in DNA demethylation through dioxygenase activity, which converts 5-methylcytosine to 5-hydroxymethylcytosine (5-hmC). However, the specific roles of TET enzymes and 5-hmC levels in head and neck squamous cell carcinoma (HNSCC) have not yet been evaluated. In this study, we analyzed 5-hmC levels and TET mRNA expression in a well-characterized dataset of 117 matched pairs of HNSCC tissues and normal tissues. 5-hmC levels and TET mRNA expression were examined via enzyme-linked immunosorbent assay and quantitative real-time PCR, respectively. 5-hmC levels were evaluated according to various clinical characteristics and prognostic implications. Notably, we found that 5-hmC levels were significantly correlated with tumor stage (P = 0.032) and recurrence (P = 0.018). Univariate analysis revealed that low levels of 5-hmC were correlated with poor disease-free survival (DFS; log-rank test, P = 0.038). The expression of TET family genes was not associated with outcomes. In multivariate analysis, low levels of 5-hmC were evaluated as a significant independent prognostic factor of DFS (hazard ratio: 2.352, 95% confidence interval: 1.136-4.896; P = 0.021). Taken together, our findings showed that reduction of TET family gene expression and subsequent low levels of 5-hmC may affect the development of HNSCC.

Keywords: 5-hmC, ELISA, TET, HNSCC, disease-free survival


Citation styles

APA
Misawa, K., Yamada, S., Mima, M., Nakagawa, T., Kurokawa, T., Imai, A., Mochizuki, D., Morita, K., Ishikawa, R., Endo, S., Misawa, Y. (2019). 5-Hydroxymethylcytosine and ten-eleven translocation dioxygenases in head and neck carcinoma. Journal of Cancer, 10(21), 5306-5314. https://doi.org/10.7150/jca.34806.

ACS
Misawa, K.; Yamada, S.; Mima, M.; Nakagawa, T.; Kurokawa, T.; Imai, A.; Mochizuki, D.; Morita, K.; Ishikawa, R.; Endo, S.; Misawa, Y. 5-Hydroxymethylcytosine and ten-eleven translocation dioxygenases in head and neck carcinoma. J. Cancer 2019, 10 (21), 5306-5314. DOI: 10.7150/jca.34806.

NLM
Misawa K, Yamada S, Mima M, Nakagawa T, Kurokawa T, Imai A, Mochizuki D, Morita K, Ishikawa R, Endo S, Misawa Y. 5-Hydroxymethylcytosine and ten-eleven translocation dioxygenases in head and neck carcinoma. J Cancer 2019; 10(21):5306-5314. doi:10.7150/jca.34806. https://www.jcancer.org/v10p5306.htm

CSE
Misawa K, Yamada S, Mima M, Nakagawa T, Kurokawa T, Imai A, Mochizuki D, Morita K, Ishikawa R, Endo S, Misawa Y. 2019. 5-Hydroxymethylcytosine and ten-eleven translocation dioxygenases in head and neck carcinoma. J Cancer. 10(21):5306-5314.

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