J Cancer 2019; 10(12):2764-2770. doi:10.7150/jca.31703
LNC CRYBG3 inhibits tumor growth by inducing M phase arrest
1. State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China
2. The Second Affiliated Hospital of Soochow University, Suzhou 215123, China
3. Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China
# These authors contributed equally.
Mao W, Guo Z, Dai Y, Nie J, Li B, Pei H, Zhou G. LNC CRYBG3 inhibits tumor growth by inducing M phase arrest. J Cancer 2019; 10(12):2764-2770. doi:10.7150/jca.31703. Available from http://www.jcancer.org/v10p2764.htm
Long noncoding RNAs (lncRNAs) are usually associated with tumor development and progression and some of them are dysregulated in various human cancers. The mechanisms underlying their dysregulation are worth further study. Here, we demonstrate that the expression level of LNC CRYBG3 is correlated with 1501 aberrantly expressed proteins in A549 cells (non-small cell lung cancer (NSCLC) cells). LNC CRYBG3 overexpression results in M phase arrest and promoted cell death, whereas LNC CRYBG3 knockdown did not elicit the opposite effects. The overexpression of LNC CRYBG3 inhibits cell proliferation both in vitro and in vivo. Moreover, it upregulates the expression of cyclin B1 and the phosphorylation of H3, whereas it inhibited the expression of cyclin-dependent kinase 6 and cyclin D1. Taken together, these findings suggest that LNC CRYBG3 regulates the cell cycle process of A549 cells, suggesting its potential application for the treatment of this disease.
Keywords: Non-small cell lung cancer, long non-coding RNA, cell cycle arrest, tumor suppressor