J Cancer 2015; 6(12):1346-1351. doi:10.7150/jca.13361

Short Research Communication

Identification of Regulatory-RNAs for Alternative Splicing of Ron Proto-Oncogene

Heegyum Moon1, Xuexiu Zheng1, Tiing Jen Loh, Ha Na Jang, Yongchao Liu, Da-Woon Jung, Darren R Williams, Haihong Shen

School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
1These authors contributed equally to this manuscript.

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Moon H, Zheng X, Loh TJ, Jang HN, Liu Y, Jung DW, Williams DR, Shen H. Identification of Regulatory-RNAs for Alternative Splicing of Ron Proto-Oncogene. J Cancer 2015; 6(12):1346-1351. doi:10.7150/jca.13361. Available from http://www.jcancer.org/v06p1346.htm

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RON receptor tyrosine kinase is a proto-oncogene that induces cell migration and matrix invasion. RONΔ160 protein, which is produced by exclusion of exon 5 and 6, promotes cell migration, matrix invasion and protection from apoptosis. Alternative splicing regulation of exon 5 and 6 is not well understood. In this manuscript, we identified several new RNA regulatory elements for alternative splicing of Ron proto-oncogene. Firstly, we demonstrated that RNA sequences from EcoRI cleavage sites regulate alternative splicing of Ron exon 5 and 6. Secondly, we showed that the ~30 nt RNA at upstream end of exon 4 and the ~33 nt RNA at downstream end of exon 7 also modulate splicing of exon 5 and 6. Thirdly, our results indicate that the RNA sequences of the ends in exon 4 and 7 are required for the regulatory functions of the RNA from restriction enzyme cleavage sites. Our results provide a new insight for regulation of alternative splicing of Ron proto-oncogene.

Keywords: Ron, pre-mRNA splicing, Regulatory-RNA sequence, Ron, exon 5, exon 6