KRT80 Promotes Lung Adenocarcinoma Progression and Serves as a Substrate for VCP

Huang, Shanhua; Tong, Weilai; Yang, Bowen; Ma, Li; Zhang, Jiaming; Wang, Chunliang; Xu, Linlin; Mei, Jinhong

doi:10.7150/jca.91753

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J Cancer 2024; 15(8):2229-2244. doi:10.7150/jca.91753 This issue Cite

Research Paper

KRT80 Promotes Lung Adenocarcinoma Progression and Serves as a Substrate for VCP

Shanhua Huang^1,2†, Weilai Tong^3†, Bowen Yang^1,2, Li Ma^1,2, Jiaming Zhang^1,2, Chunliang Wang⁴, Linlin Xu^1,2✉, Jinhong Mei^1,2✉

1. Department of Pathology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China.
2. Institute of Molecular Pathology, Jiangxi Medical College, Nanchang University, Nanchang, China.
3. Department of Orthopedics, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China.
4. Department of Neurosurgery, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China.
†These authors contributed equally to this work and share first authorship.

Citation:

Huang S, Tong W, Yang B, Ma L, Zhang J, Wang C, Xu L, Mei J. KRT80 Promotes Lung Adenocarcinoma Progression and Serves as a Substrate for VCP. J Cancer 2024; 15(8):2229-2244. doi:10.7150/jca.91753. https://www.jcancer.org/v15p2229.htm

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Abstract

Background: Keratin 80(KRT80) encodes a type II intermediate filament protein, known for maintaining cell integrity of cells and its involvement in the tumorigenesis and progression of various cancers. However, comprehensive research on its relevance to lung adenocarcinoma remains limited.

Methods: In this study, we utilized multiple databases to investigate the transcriptional expression of KRT80 and its correlation with clinicopathological features. A range of assays, including the Cell Counting Kit 8 assay, colony formation assay, cell migration assay, and flow cytometry, were employed to elucidate the impact of KRT80 on the malignant behavior of lung adenocarcinoma. Immunoprecipitation and mass spectrometry were also used to identify putative genes interacting with KRT80.

Results: The expression of KRT80 was elevated in lung adenocarcinoma and patients with high levels of KRT80 expression had poor clinical outcomes. Silencing KRT80 suppressed cell viability, and migration, while overexpression had the opposite effect. In addition, Immunoprecipitation and mass spectrometry revealed an interaction between KRT80 and valosin-containing protein (VCP), with VCP knockdown reducing the stability of KRT80 protein. Overexpression of KRT80 mitigated the inhibitory effect of VCP knockdown to some extent.

Conclusion: Our findings collectively suggest that KRT80 is a promising diagnostic and prognostic indicator for lung adenocarcinoma. Additionally, the interaction between KRT80 and VCP plays a crucial role in the progression of lung adenocarcinoma, which implies that KRT80 is a promising therapeutic target.

Keywords: lung adenocarcinoma, KRT80, VCP, PI3K/AKT pathway, progression

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APA

Huang, S., Tong, W., Yang, B., Ma, L., Zhang, J., Wang, C., Xu, L., Mei, J. (2024). KRT80 Promotes Lung Adenocarcinoma Progression and Serves as a Substrate for VCP. Journal of Cancer, 15(8), 2229-2244. https://doi.org/10.7150/jca.91753.

ACS

Huang, S.; Tong, W.; Yang, B.; Ma, L.; Zhang, J.; Wang, C.; Xu, L.; Mei, J. KRT80 Promotes Lung Adenocarcinoma Progression and Serves as a Substrate for VCP. J. Cancer 2024, 15 (8), 2229-2244. DOI: 10.7150/jca.91753.

NLM

CSE

Huang S, Tong W, Yang B, Ma L, Zhang J, Wang C, Xu L, Mei J. 2024. KRT80 Promotes Lung Adenocarcinoma Progression and Serves as a Substrate for VCP. J Cancer. 15(8):2229-2244.

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