J Cancer 2021; 12(23):7130-7137. doi:10.7150/jca.60554 This issue

Research Paper

Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions

Jiang Zhu1#, Lingjing Chen2#, Qiaoli Zheng1, Rui Han1, Xianzhen Chen1, Qiang Zhou1, Hao Cheng1✉

1. Department of Dermatology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, Zhejiang Province, China.
2. Department of Dermatology, Hangzhou Children's Hospital, Hangzhou 310014, Zhejiang Province, China.
#These authors contributed equally to this work.

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Citation:
Zhu J, Chen L, Zheng Q, Han R, Chen X, Zhou Q, Cheng H. Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions. J Cancer 2021; 12(23):7130-7137. doi:10.7150/jca.60554. Available from https://www.jcancer.org/v12p7130.htm

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Abstract

Graphic abstract

Almost all cervical cancer is associated with persistent infection of high-risk (hr) human papillomavirus (HPV) like HPV16. The E7 oncoprotein encoded by hrHPV plays a crucial role in carcinogenesis. The present study aimed to establish a reliable protocol of immunohistochemistry stains to detect HPV16 E7 protein in formalin-fixed and paraffin-embedded tissue specimens of various cervical lesions. Firstly, the HPV16 E7 gene was inserted into a prokaryotic expression vector pGEX-4T2. Then the recombinant plasmid pGEX-4T2-(HPV16-E7) was transformed into Escherichia coli JM109. The fusion protein containing a GST tag was purified, and New Zealand white rabbits were immunized to produce the HPV16 E7 polyclonal antibody. The anti-HPV16 E7 antibody was verified by western blotting and immunofluorescence stains, and applied in 182 HPV16 DNA-positive cervical tissue specimens and matched 36 HPV DNA-negative specimens by immunohistochemistry. Furthermore, A positive correlation between HPV16 E7 protein expression and malignancy grade was observed. But there is no relationship between HPV16 E7 protein expression and tumor sizes, tumor differentiation, lymph node metastasis, International Federation of Gynecology and Obstetrics (FIGO) stage, or lymphovascular space invasion in cervical cancer. These findings provide a basis for further research focusing on the role of HPV E7 protein in various HPV-related diseases.

Keywords: Human papillomavirus (HPV), E7 protein, Polyclonal antibody, Immunohistochemistry, Cervical cancer