J Cancer 2021; 12(16):4924-4932. doi:10.7150/jca.49942

Research Paper

circALPL Sponges miR-127 to Promote Gastric Cancer Progression by Enhancing MTDH Expression

Peng Wu2, Dongmei Ye3, Jiaoyan Li3, Fei Yan3, Xin Jin3, Zhiwei Zhang3, Zhenfa Li1✉

1. Affiliated Hengyang Hospital, Southern Medical University (Hengyang Central Hospital), 12# Yancheng road, Hengyang 421001, Hunan province, China.
2. Department of Critical Care Medicine, Hengyang Maternal and Child Health Hospital, Hengyang, 421001, Hunan Province, China.
3. Cancer Research Institute of Hengyang Medical College, University of South China; Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan, 28 Changsheng Road, Hengyang, Hunan 421001, Hunan Province, China.

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Citation:
Wu P, Ye D, Li J, Yan F, Jin X, Zhang Z, Li Z. circALPL Sponges miR-127 to Promote Gastric Cancer Progression by Enhancing MTDH Expression. J Cancer 2021; 12(16):4924-4932. doi:10.7150/jca.49942. Available from https://www.jcancer.org/v12p4924.htm

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Abstract

Background: CircRNA plays an important role in cancer progression. However, the potential mechanism of circRNA in gastric cancer remains unknown. In this study, we aimed to investigate the specific mechanism of circALPL in gastric cancer.

Methods: Using a high-throughput microarray, we found that circALPL was upregulated in gastric cancer cell lines. RT-qPCR was used to measure the circALPL expression level in gastric cell lines and tissue. Transwell, CCK-8, and metastasis assays were performed to learn the function after circALPL was inhibited.

Results: circALPL downregulation suppresses the invasion and proliferation ability of gastric cancer cells. Additionally, the underlying pathway of circALPL was studied using luciferase reporter assays and RNA immunoprecipitation assays. The results showed that circALPL promotes gastric cancer progression by sponging miR-127, thus upregulating MTDH.

Conclusion: The circALPL-miR-127-MTDH pathway plays a vital role in gastric cancer proliferation and metastasis. circALPL might be a new therapeutic target in gastric cancer.

Keywords: circALPL, gastric cancer, MTDH, microRNA, competitive endogenous RNAs