Full Text | PDF

J Cancer 2021; 12(13):4049-4063. doi:10.7150/jca.54221 This issue Cite

Research Paper

Alterations of bacterial communities of vocal cord mucous membrane increases the risk for glottic laryngeal squamous cell carcinoma

Zhen Dong^1,2,3, Chunming Zhang^1,2,3, Qinli Zhao^2,3, Hui Huangfu^2,3, Xuting Xue^1,3, Shuxin Wen^4,3, Yongyan Wu^1,3,5,6✉, Wei Gao^1,3,5,7✉, Binquan Wang^1,2,3✉

1. Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
2. Department of Otolaryngology Head & Neck Surgery, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
3. Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
4. Department of Otolaryngology Head & Neck Surgery, Shanxi Bethune Hospital, Taiyuan 030032, China.
5. Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, 030001, China.
6. Department of Biochemistry & Molecular Biology, Shanxi Medical University, Taiyuan 030001, China.
7. Department of Cell biology and Genetics, Basic Medical School, Shanxi Medical University, Taiyuan 030001, China.

✉ Corresponding authors: Binquan Wang, M.D., Prof., Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, No.86, Jiefang Road South, Taiyuan 030001, China, E-mail: wbq_xyorg, ORCID:0000-0002-3686-0219; Wei Gao, M.D., Prof., Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, No.86, Jiefang Road South, Taiyuan 030001, China, E-mail: gaoweisxentorg, ORCID:0000-0001-7836-2851; Yongyan Wu, Ph.D., Prof., Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, No.86, Jiefang Road South, Taiyuan 030001, China, E-mail: wuyongyanorg, ORCID:0000-0003-1669-3860. More

Abstract

Bacteria are among the important factors that play a role in the balance of human health, and their relationship with some tumors has been well established. However, the association between bacteria colonizing the vocal cords and glottic laryngeal squamous cell carcinoma (GLSCC) remains unclear. Here, we investigated whether bacterial communities of the vocal cord mucous membrane play a role in the development of GLSCC. We collected tumor tissue and normal adjacent tissue (NAT) samples from 19 GLSCC patients, and the bacterial communities were compared with control samples (control) from 21 vocal cord polyps using 16S rRNA high-throughput pyrosequencing. We detected 41 phyla, 93 classes, 188 orders, 373 families, and 829 genera in the vocal cord mucous membrane. A comparison of the bacterial communities in the NAT samples showed higher α‐diversity than in the tumor samples. In the tumor samples, seven groups of bacteria, i.e., the phylum Fusobacteria, the class Fusobacteriia, the order Fusobacteriales, the family Fusobacteriaceae, and the genera Fusobacterium, Alloprevotella, and Prevotella, were significantly enriched, as revealed by linear discriminant analysis coupled with effect size measurements (LEfSe). However, bacteria from the phylum Firmicutes were most significantly enriched in the vocal cord polyp tissues. These findings suggest alterations in the bacterial community structure of the vocal cord mucous membrane of GLSCC patients and that seven groups of bacteria are related to GLSCC, indicating that imbalances in bacterial communities increase the risk for the development of GLSCC.

Keywords: microbiota, bacterial communities, vocal cord, glottic laryngeal squamous cell carcinoma, 16S rRNA sequences.

Citation styles

©2024 Ivyspring International Publisher. Terms of use