J Cancer 2021; 12(11):3249-3256. doi:10.7150/jca.55558 This issue

Research Paper

Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p

Jin Qian1✉, Yingna Xu1, Xing Xu1, Zhenyu Tao1, Yang Luo1, Yichang Xu1, Yong Zhang1, Chunfa Qian2✉

1. Department of Neurosurgery, People's Hospital of Xuancheng City, Xuancheng, Anhui, China.
2. Department of Neurosurgery, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Qian J, Xu Y, Xu X, Tao Z, Luo Y, Xu Y, Zhang Y, Qian C. Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p. J Cancer 2021; 12(11):3249-3256. doi:10.7150/jca.55558. Available from https://www.jcancer.org/v12p3249.htm

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Graphic abstract

Glioma is a pervasive malignancy and the main cause of cancer-related deaths worldwide. Circular RNA is an important subject of cancer research, and its role and function in glioma are poorly understood. This study demonstrated that hsa_circ_0091581 is upregulated in glioma tissues and cells. The results of the CCK-8, EdU, and transwell assays indicated that hsa_circ_0091581 promotes proliferation, migration, and invasion of glioma cells. The results of the luciferase reporter and RNA immunoprecipitation assays indicated that the mechanism of the effects of hsa_circ_0091581 on glioma cells involves sponging miR-1243-5p to regulate RMI1. The results of the rescue experiments indicated that hsa_circ_0091581 regulates proliferation, migration, and invasion of glioma cells by targeting RMI1 in a miR-1243-5p dependent manner. The results of the nude mice xenograft assays showed that knockdown of hsa_circ_0091581 inhibits glioma growth in vivo. Thus, our study determined the role of hsa_circ_0091581/miR-1243-5p/RMI1 in glioma and suggests that this axis may be a novel therapeutic target in glioma.

Keywords: circ_0091581, miR-1243-5p, RMI1, glioma.