Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p

Jin Qian^1✉, Yingna Xu¹, Xing Xu¹, Zhenyu Tao¹, Yang Luo¹, Yichang Xu¹, Yong Zhang¹, Chunfa Qian^2✉

1. Department of Neurosurgery, People's Hospital of Xuancheng City, Xuancheng, Anhui, China.
2. Department of Neurosurgery, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

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Citation:
Qian J, Xu Y, Xu X, Tao Z, Luo Y, Xu Y, Zhang Y, Qian C. Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p. J Cancer 2021; 12(11):3249-3256. doi:10.7150/jca.55558. Available from https://www.jcancer.org/v12p3249.htm

Abstract

Glioma is a pervasive malignancy and the main cause of cancer-related deaths worldwide. Circular RNA is an important subject of cancer research, and its role and function in glioma are poorly understood. This study demonstrated that hsa_circ_0091581 is upregulated in glioma tissues and cells. The results of the CCK-8, EdU, and transwell assays indicated that hsa_circ_0091581 promotes proliferation, migration, and invasion of glioma cells. The results of the luciferase reporter and RNA immunoprecipitation assays indicated that the mechanism of the effects of hsa_circ_0091581 on glioma cells involves sponging miR-1243-5p to regulate RMI1. The results of the rescue experiments indicated that hsa_circ_0091581 regulates proliferation, migration, and invasion of glioma cells by targeting RMI1 in a miR-1243-5p dependent manner. The results of the nude mice xenograft assays showed that knockdown of hsa_circ_0091581 inhibits glioma growth in vivo. Thus, our study determined the role of hsa_circ_0091581/miR-1243-5p/RMI1 in glioma and suggests that this axis may be a novel therapeutic target in glioma.

Keywords: circ_0091581, miR-1243-5p, RMI1, glioma.