J Cancer 2021; 12(6):1722-1728. doi:10.7150/jca.50816

Research Paper

HPV58 E7 Protein Expression Profile in Cervical Cancer and CIN with Immunohistochemistry

Qiaoli Zheng1, Xianzhen chen1, Rui Han1, Jiang Zhu1, Hui Wang2, Lingjing Chen3, Yinjing Song1, Luxia Chen1, Hao Cheng1✉, Na Jin1✉

1. Department of Dermatology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
2. Department of Pathology, The First People's Hospital of Fuyang, Hangzhou, Zhejiang Province, China.
3. Department of Dermatology, Hangzhou Children's Hospital, Hangzhou, Zhejiang Province, China.

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Citation:
Zheng Q, chen X, Han R, Zhu J, Wang H, Chen L, Song Y, Chen L, Cheng H, Jin N. HPV58 E7 Protein Expression Profile in Cervical Cancer and CIN with Immunohistochemistry. J Cancer 2021; 12(6):1722-1728. doi:10.7150/jca.50816. Available from https://www.jcancer.org/v12p1722.htm

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Abstract

Background: The persistent infection of high-risk human papillomavirus (HR-HPV) is one of the most common causes of cervical cancer worldwide, and HPV type 58 (HPV58) is the third most common HPV type in eastern Asia. The E7 oncoprotein is constitutively expressed in HPV58-associated cervical cancer cells and plays a key role during tumorigenesis. This study aimed to assess the HPV58 E7 protein expression in the tissues of cervical cancer and cervical intraepithelial neoplasia (CIN).

Methods: A total of 67 HPV58-positive cervical samples were collected, including 25 cervical cancer samples and 42 CIN samples. All the tissues were examined by HPV58 E7, p16INK4a and Ki67 immunohistochemistry (IHC). At last, we analyzed their association with clinical and pathological variables.

Results: HPV58 E7 expression was detected in 96% of the HPV58 DNA-positive cervical cancer tissues and 85.7% of HPV58-positive CIN tissues. 65 samples of cervical cancer and CIN tissues had p16-positive staining, while 59 samples were Ki-67 positive.

Conclusions: HPV58 E7 protein is highly expressed in both cervical cancer and CIN tissues. HPV58 E7 IHC could be sensitive and specific for evaluating HPV-driven cervical cancer and pre-cancerous lesions, in combination with p16 and Ki-67 IHC.

Keywords: cervical cancer, CIN, E7, HR-HPV, immunohistochemistry