Long Noncoding RNA FAM225A Promotes Esophageal Squamous Cell Carcinoma Development and Progression via Sponging MicroRNA-197-5p and Upregulating NONO

Zhu, Pengyuan; Huang, Haitao; Gu, Shaorui; Liu, Zhenchuan; Zhang, Xin; Wu, Kaiqin; Lu, Tiancheng; Li, Lei; Dong, Chenglai; Zhong, Chongjun; Zhou, Yongxin

doi:10.7150/jca.51292



International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

Global reach, higher impact

Full Text | PDF

J Cancer 2021; 12(4):1073-1084. doi:10.7150/jca.51292 This issue Cite

Research Paper

Long Noncoding RNA FAM225A Promotes Esophageal Squamous Cell Carcinoma Development and Progression via Sponging MicroRNA-197-5p and Upregulating NONO

Pengyuan Zhu¹, Haitao Huang², Shaorui Gu², Zhenchuan Liu², Xin Zhang², Kaiqin Wu², Tiancheng Lu², Lei Li², Chenglai Dong², Chongjun Zhong^1✉, Yongxin Zhou^2✉

1. Department of thoracic and Cardiovascular Surgery, the Second Affiliated Hospital of Nantong University, School of Medicine, Nantong University, Nantong, 226001, China.
2. Department of thoracic and Cardiovascular Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China.

Citation:

Zhu P, Huang H, Gu S, Liu Z, Zhang X, Wu K, Lu T, Li L, Dong C, Zhong C, Zhou Y. Long Noncoding RNA FAM225A Promotes Esophageal Squamous Cell Carcinoma Development and Progression via Sponging MicroRNA-197-5p and Upregulating NONO. J Cancer 2021; 12(4):1073-1084. doi:10.7150/jca.51292. https://www.jcancer.org/v12p1073.htm

Other styles

Abstract

Esophageal squamous cell carcinoma (ESCC) is the major subclass of esophageal cancer and one of the most life-threatening malignancies with high morbidity and mortality. Long noncoding RNAs (lncRNAs) participate in tumorigenesis and metastasis of various tumors. Here, we investigated the function of a newly identified lncRNA FAM225A in ESCC. LncRNA FAM225A expression was significantly higher in ESCC and predicted poor prognosis of ESCC patients. We confirmed that upregulation of FAM225A in ESCC and overexpression of FAM225A was associated with poor outcome in ESCC patients using TCGA ESCC cohort. Knockdown of FAM225A significantly inhibited cell growth, migration and invasion of ESCC cells in vitro and inhibited ESCC xenograft development in vivo. Mechanistically, we demonstrated that lncRNA FAM225A functioned as a competing endogenous RNA (ceRNA) via sponging miR-197-5p. LncRNA FAM225A exerted its regulatory function on ESCC proliferation and metastasis via modulating expression of miR-197-5p. MiR-197-5p overexpression antagonized the function of FAM225A, with decreased cell growth and invasion. Moreover, we identified that RNA binding protein NONO was a direct target of miR-197-5p and miR-197-5p negatively regulated NONO expression and TGF-β signaling in ESCC cells. In summary, our findings suggest that lncRNA FAM225A promotes ESCC development and progression via sponging miR-197-5p and upregulating NONO expression. These results suggest that lncRNA FAM225A could be explored as a new therapy target in ESCC treatment.

Keywords: FAM225A, microRNA-197-5p, NONO, ESCC

Citation styles

APA

Zhu, P., Huang, H., Gu, S., Liu, Z., Zhang, X., Wu, K., Lu, T., Li, L., Dong, C., Zhong, C., Zhou, Y. (2021). Long Noncoding RNA FAM225A Promotes Esophageal Squamous Cell Carcinoma Development and Progression via Sponging MicroRNA-197-5p and Upregulating NONO. Journal of Cancer, 12(4), 1073-1084. https://doi.org/10.7150/jca.51292.

ACS

Zhu, P.; Huang, H.; Gu, S.; Liu, Z.; Zhang, X.; Wu, K.; Lu, T.; Li, L.; Dong, C.; Zhong, C.; Zhou, Y. Long Noncoding RNA FAM225A Promotes Esophageal Squamous Cell Carcinoma Development and Progression via Sponging MicroRNA-197-5p and Upregulating NONO. J. Cancer 2021, 12 (4), 1073-1084. DOI: 10.7150/jca.51292.

NLM

CSE

Zhu P, Huang H, Gu S, Liu Z, Zhang X, Wu K, Lu T, Li L, Dong C, Zhong C, Zhou Y. 2021. Long Noncoding RNA FAM225A Promotes Esophageal Squamous Cell Carcinoma Development and Progression via Sponging MicroRNA-197-5p and Upregulating NONO. J Cancer. 12(4):1073-1084.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.