J Cancer 2020; 11(19):5727-5737. doi:10.7150/jca.46554

Research Paper

The diagnostic and prognostic value of cell division cycle associated gene family in Hepatocellular Carcinoma

Bowen Wu1*, Yu Huang2*, Yingwan Luo3, An Ma4, Zhaoxing Wu1, Yichao Gan1, Ying Xu1, Rongzhen Xu1,5✉

1. Department of Hematology (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences, Zhejiang Province) and Cancer Institute, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310009, China.
2. Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
3. Divisions of Pathology and Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, USA.
4. Zhejiang Academy of Medical Sciences, Hangzhou 310009, China.
5. Institute of Hematology, Zhejiang University, Hangzhou, 310009, China.
*These authors contribute equally to this work.

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Wu B, Huang Y, Luo Y, Ma A, Wu Z, Gan Y, Xu Y, Xu R. The diagnostic and prognostic value of cell division cycle associated gene family in Hepatocellular Carcinoma. J Cancer 2020; 11(19):5727-5737. doi:10.7150/jca.46554. Available from http://www.jcancer.org/v11p5727.htm

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Cell division cycle associated (CDCA) gene family plays an important role in cells. However, some researchers revealed that overexpression of CDCAs might contribute to the tumor progression in several cancers. Here, we analyzed the role of this gene family in hepatocellular carcinoma (HCC). We used several web tools and found that most of CDCAs were highly expressed in tumor tissues compared to the paracancer tissues in HCC. We then used RT-qPCR to confirm our results. The results showed that CDCA2, CDCA3, CDCA5 and CDCA8 were up-regulated in HCC. We also found that these genes were associated with poor overall survival and relapse free survival except CDCA7. The functional analysis showed that this gene family might take part in many processes, including cell division, apoptosis, DNA damage and DNA repair, which might contribute to the tumor progression. The KEGG pathway analysis showed that these genes participated in several important pathways such as PI3K-Akt signaling pathway and hippo signaling pathway. In conclusion, our findings suggested that CDCA2, CDCA3, CDCA4, CDCA5, and CDCA8 might have potential diagnostic and prognostic values for hepatocellular carcinoma.

Keywords: cell division cycle, gene family, hepatocellular carcinoma