J Cancer 2020; 11(19):5612-5622. doi:10.7150/jca.46097

Research Paper

ALKBH5 regulates IGF1R expression to promote the Proliferation and Tumorigenicity of Endometrial Cancer

Xiaowen Pu1#, Zhuowei Gu2#, Zhengrong Gu1✉

1. Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 201204, China.
2. Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
#These authors contributed equally to this work.

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Citation:
Pu X, Gu Z, Gu Z. ALKBH5 regulates IGF1R expression to promote the Proliferation and Tumorigenicity of Endometrial Cancer. J Cancer 2020; 11(19):5612-5622. doi:10.7150/jca.46097. Available from http://www.jcancer.org/v11p5612.htm

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Abstract

N6-methyladenosine (m6A) messenger RNA methylation play important role in cell proliferation and tumorigenicity of endometrial cancer, but the key mechanism is not fully clear. Here, we found that RNA demethylase ALKBH5 expression was significantly upregulated in endometrial cancer, ALKBH5 was then identified to positively regulate proliferation and invasion of endometrial cancer. Mechanistically, the m6A eraser ALKBH5 demethylated target transcripts IGF1R and enhanced IGF1R mRNA stability, consequently promoting IGF1R translation and activating IGF1R signaling pathway. Thus, we demonstrated that ALKBH5 promoted proliferation and invasion of endometrial cancer via erasing IGF1R m6A-modifications, which suggests a potential therapeutic target for endometrial cancer.

Keywords: ALKBH5, IGF1R, Endometrial cancer, Insulin signaling pathway, m6A modification