J Cancer 2020; 11(17):5088-5098. doi:10.7150/jca.44777

Research Paper

Knockdown of Kif20a inhibits growth of tumors in soft tissue sarcoma in vitro and in vivo

Zhenhua Zhu1, Zheng Jin2, Haibo Zhang3, Mei Zhang3✉*, Dahui Sun1✉*

1. Department of Orthopaedic Trauma, The First Hospital of Jilin University, Xinmin Street 71#, Changchun City, Jilin Province, China.
2. Department of Immunology, College of Basic Medical Sciences, Jilin University, Xinmin Street 126#, Changchun City, Jilin Province, China.
3. College of Chemistry, Jilin University, Changchun, Jilin Province, China.
*Equal contributions to this work.

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Citation:
Zhu Z, Jin Z, Zhang H, Zhang M, Sun D. Knockdown of Kif20a inhibits growth of tumors in soft tissue sarcoma in vitro and in vivo. J Cancer 2020; 11(17):5088-5098. doi:10.7150/jca.44777. Available from https://www.jcancer.org/v11p5088.htm

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Abstract

Kif20a (Kinesin Family Member 20A), plays a role in cell mitosis, cell migration and intracellular transport. Numerous studies have demonstrated that Kif20a is abnormally highly expressed in a variety of tumors and shows poor prognosis. Soft tissue sarcoma (STS) represents a group of malignant tumors with poor prognosis. The role of Kif20a in STSs has not been systematically studied. In the present study, bioinformatics analysis, in vitro and in vivo experiments were conducted to investigate the function of Kif20a in STSs. In bioinformatics analysis higher KIf20a expression indicated a poor prognosis. Functional enrichment analysis indicated that Kif20a may be related to cell cycle, p53 and other signaling pathways. In vitro experiments showed that after the down-regulation of Kif20a, cell proliferation, migration and invasion were decreased, while apoptosis was increased. In vivo experiments revealed that Kif20a affected the proliferation of tumors in tumor-bearing mice. In summary, our findings revealed that Kif20a performs an important role in STS, indicating that it is a potential prognostic biomarker and potentially representing a therapeutic target for the disease.

Keywords: Kif20a, bioinformatics, survival, biomarkers, soft tissue sarcoma