J Cancer 2020; 11(12):3551-3558. doi:10.7150/jca.40010

Research Paper

Prognostic Value of ANXA8 in Gastric Carcinoma

Fangqi Ma1#, Xiaowei Li1#, Haiming Fang3, Yueping Jin2, Qin Sun2, Xuejun Li2✉

1. The Graduate School, Anhui University of Traditional Chinese Medicine, Hefei, Anhui, China.
2. Department of Gastroenterology, The Second Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, Anhui, China.
3. Department of Gastroenterology, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
#These authors contributed equally to this work.

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Citation:
Ma F, Li X, Fang H, Jin Y, Sun Q, Li X. Prognostic Value of ANXA8 in Gastric Carcinoma. J Cancer 2020; 11(12):3551-3558. doi:10.7150/jca.40010. Available from http://www.jcancer.org/v11p3551.htm

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Abstract

Gastric carcinoma (GC) remains one of the most common and deadly cancers worldwide. In China, the incidence and mortality rates related to GC were quite high. Annexin A8 (ANXA8) is a member of the annexins family of calcium-dependent membrane phospholipid binding proteins. According to recent research, the up-regulation of ANXA8 is closely associated with various types of tumors. However, the specific role of ANXA8 in GC remains unclear. In our study, we explored the prognostic value of ANXA8 in GC. Here, with the data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets (GSE19826 and GSE13861) analyzed, we further performed quantitative real-time polymerase chain reaction (qRT-PCR) using 58 pairs of fresh-frozen tissues. We also subjected 152 pairs of formalin-fixed, paraffin-embedded GC tumor tissues from patients, and the adjacent normal gastric tissues (ANGTs) to immunohistochemical (IHC) analysis. Hence, we found an elevated expression of ANXA8 in tumor tissues with bioinformatics analyses, qRT-PCR, western blot and IHC. Over-expression of ANXA8 was strongly correlated with TNM stages and differentiation grades. Kaplan-Meier and cox proportional-hazard analyses showed that the increased expression of ANXA8 was strongly associated with overall survival (OS) and disease-free survival (DFS) in GC patients. Moreover, we found that ANXA8 is an independent prognostic factor of GC patients' OS and DFS. In brief, those results suggest that ANXA8 can act as an oncogene of GC development and can serve as a potential prognostic biomarker for GC treatment.

Keywords: Gastric carcinoma (GC), Annexin A8 (ANXA8), Biomarker, Independent prognostic factor