J Cancer 2019; 10(12):2783-2787. doi:10.7150/jca.31450

Research Paper

The characteristics of HPV integration in cervical intraepithelial cells

Weiping Li1, Shuang Tian1, Pengpeng Wang2, Yikun Zang2, Xin Chen3, Yuanqing Yao1✉, Weiyang Li4✉

1. Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, P. R. China
2. MyGenostics Inc., Beijing, P. R. China
3. ME Genomics Inc., Shenzhen, P. R. China
4. Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province, Jining Medical University, Jining, Shandong 272067, China

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Citation:
Li W, Tian S, Wang P, Zang Y, Chen X, Yao Y, Li W. The characteristics of HPV integration in cervical intraepithelial cells. J Cancer 2019; 10(12):2783-2787. doi:10.7150/jca.31450. Available from http://www.jcancer.org/v10p2783.htm

File import instruction

Abstract

Cervical cancer is one of the most common malignant tumors in gynecology. Deploying HIVID, a cost-effective technique to detect HPV integration sites, our team had studied the characteristics of HPV integrations in cervical exfoliated cells. Our results indicated that both the sample proportion and the number of HPV integrations gradually increased following the development of cervical lesion. Meanwhile, our data also revealed that there were recurrent genes integrated by HPV in cervical exfoliated cells. Collectively, the HPV integration breakpoints were highly enriched in the intron and promoter regions. Intriguingly, the gene pathway analysis indicated that the HPV-integrated genes were strongly inclined to pathways of metabolism of xenobiotics by cytochrome P450, chemical carcinogenesis and steroid hormone biosynthesis. In conclusion, this study unveiled the HPV integration patterns and the associated recurrent genes in cervical epithelial exfoliated cells. Altogether, our data suggested that the HPV integrations in cervical exfoliated cells might have vital clinical significance, and probably also diagnostic and/or prognostic values in future clinical applications.

Keywords: HPV integration, Cervical exfoliated cells, Cervical cancer, Characteristics