J Cancer 2018; 9(4):745-756. doi:10.7150/jca.21614
Biological Function of Ribosomal Protein L10 on Cell Behavior in Human Epithelial Ovarian Cancer
1. Center Laboratory, Jinshan Hospital, Fudan University, Shanghai 201508, China;
2. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
* These authors contributed equally to this work.
Shi J, Zhang L, Zhou D, Zhang J, Lin Q, Guan W, Zhang J, Ren W, Xu G. Biological Function of Ribosomal Protein L10 on Cell Behavior in Human Epithelial Ovarian Cancer. J Cancer 2018; 9(4):745-756. doi:10.7150/jca.21614. Available from http://www.jcancer.org/v09p0745.htm
Ribosomal protein L10 (RPL10) is one of large ribosomal proteins and plays a role in Wilms' tumor and premature ovarian failure. However, the function of RPL10 in human epithelial ovarian cancer (EOC) remains unknown. The purpose of this study was to examine the expression level and function of RPL10 in EOC. RPL10 protein expression was detected by immunohistochemistry and Western blot. The association RPL10 expression with clinical features was analyzed. Loss-of-function and gain-of-function approaches were applied in cellular assays, including cell viability, migration, invasion, and apoptosis. Our study demonstrated for the first time that RPL10 was upregulated in human EOC compared with normal ovarian tissues. Knockdown of RPL10 inhibited cell viability, migration, and invasion, and increased cell apoptosis. On the contrary, upregulation of RPL10 increased cell viability, migration, invasion, and decreased cell apoptosis. Furthermore, miR-143-3p regulated RPL10 expression. Our data indicate that RPL10 is a potential tissue biomarker of patients with EOC and may be a therapeutic target of ovarian cancer.
Keywords: Cell biology, biomarker, ovarian cancer, QM, RPL10, therapeutic target