1. Cancer Center, the First Hospital of Jilin University, No. 71. Xinmin Street, Changchun, 130021, China; 2. Pathological diagnostic Center, the First Hospital of Jilin University, No.71.Xinmin Street, Changchun, 130021, China; 3. Stanford University Medical School, VA Palo Alto Health Care System, Palo Alto, CA 94304, USA.
✉ Corresponding authors: Jiuwei Cui, Cancer Center, the First Hospital of Jilin University, No.71, Xinmin Street, Changchun, 130021, China, Tel: 86-43188782178, Fax: 86-43188786134, e-mail: cuijwedu.cn; or Ji-Fan Hu, Stanford University Medical School, Palo Alto Veterans Institute for Research, Palo Alto, CA 94304, USA, Tel: 650-493-5000, x63175, Fax: 650-849-1213, e-mail: jifanedu.More
Citation:
Pang J, Yan X, Cao H, Qian L, He H, Tian H, Han F, Wang G, Chen X, Zhao Y, Hu JF, Cui J. Knockdown of COPS3 Inhibits Lung Cancer Tumor Growth in Nude Mice by Blocking Cell Cycle Progression. J Cancer 2017; 8(7):1129-1136. doi:10.7150/jca.16201. https://www.jcancer.org/v08p1129.htm
COPS3 encodes the third subunit of the COP9 signalosome and its aberrant expression is associated with many RITE (“Region of Increased Tumor Expression”) genes in lung cancer tissues. To elucidate the specific role of COPS3 in lung cancer, we examined its expression in lung cancer tissues by immunohistochemical staining. We found that COPS3 was overexpressed in most of the lung cancer samples examined, particularly in small cell carcinoma and squamous cell carcinoma. The expression of COPS3 protein was positively correlated with the level of Ki-67 cell proliferation index (p=0.001) and negatively related to the degree of tumor differentiation (p=0.012). In a xenograft tumor model in nude mice, shRNA-knockdown of COPS3 significantly reduced tumor growth. In lung adenocarcinoma A549 cells, shRNA-knockdown of COPS3 induced cell cycle arrest at G0/G1 phase by upregulating the cell cycle regulator protein P21 and downregulating cyclin B1 and CDK4. These data suggest that COPS3 may promote tumor growth by regulating cell-cycle associated proteins.
Pang, J., Yan, X., Cao, H., Qian, L., He, H., Tian, H., Han, F., Wang, G., Chen, X., Zhao, Y., Hu, J.F., Cui, J. (2017). Knockdown of COPS3 Inhibits Lung Cancer Tumor Growth in Nude Mice by Blocking Cell Cycle Progression. Journal of Cancer, 8(7), 1129-1136. https://doi.org/10.7150/jca.16201.
ACS
Pang, J.; Yan, X.; Cao, H.; Qian, L.; He, H.; Tian, H.; Han, F.; Wang, G.; Chen, X.; Zhao, Y.; Hu, J.F.; Cui, J. Knockdown of COPS3 Inhibits Lung Cancer Tumor Growth in Nude Mice by Blocking Cell Cycle Progression. J. Cancer 2017, 8 (7), 1129-1136. DOI: 10.7150/jca.16201.
NLM
Pang J, Yan X, Cao H, Qian L, He H, Tian H, Han F, Wang G, Chen X, Zhao Y, Hu JF, Cui J. Knockdown of COPS3 Inhibits Lung Cancer Tumor Growth in Nude Mice by Blocking Cell Cycle Progression. J Cancer 2017; 8(7):1129-1136. doi:10.7150/jca.16201. https://www.jcancer.org/v08p1129.htm
CSE
Pang J, Yan X, Cao H, Qian L, He H, Tian H, Han F, Wang G, Chen X, Zhao Y, Hu JF, Cui J. 2017. Knockdown of COPS3 Inhibits Lung Cancer Tumor Growth in Nude Mice by Blocking Cell Cycle Progression. J Cancer. 8(7):1129-1136.
This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.