J Cancer 2017; 8(5):887-893. doi:10.7150/jca.17747 This issue Cite

Research Paper

Aided Diagnosis of Hepatocellular Carcinoma Using Serum Fucosylated Haptoglobin Ratios

Shuxin Shang1, 2, Wei Li3, Xue Qin2✉, Shu Zhang1✉, Yinkun Liu1, 3

1. Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, China;
2. Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China;
3. Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

Citation:
Shang S, Li W, Qin X, Zhang S, Liu Y. Aided Diagnosis of Hepatocellular Carcinoma Using Serum Fucosylated Haptoglobin Ratios. J Cancer 2017; 8(5):887-893. doi:10.7150/jca.17747. https://www.jcancer.org/v08p0887.htm
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Abstract

Aberrant fucosylation plays a functional role in regulating ontogeny and celluar differentiation and are differentially regulated in cancerous condition, which could provide hallmarks for cancer diagnostics and surveillance. We previously developed a magnetic beads-based lectin ELISA system to measure fucosylated haptoglobin (Hp), which has been reported to be a cancer biomarker through a series of glycoproteomic analysis. In this study, serum fucosylated Hp ratios were measured using our ELISA kit in a separate cohort of 260 patients independently, including 130 healthy controls and 130 patients with hepatocellular carcinoma (HCC). Fucosylated Hp /Hp ratio (levels of fucosylated Hp /levels of protein Hp) and ELISA Index (OD value of fucosylated Hp /OD value of protein Hp) were calculated respectively to reflect Hp fucosylation level on its protein level. Our data showed that fucosylated Hp /Hp ratio (AUC=0.8449) and ELISA Index (AUC=0.8581) had better performance in distinguishing HCC from controls, which indicated that fucosylated Hp ratios could improve the diagnosis and prediction of HCC even with a low level of alpha-fetoprotein (AFP). Additionally, the combination analysis of AFP and fucosylated Hp ratios increased the AUC value for HCC diagnosis.

Keywords: biomarker, fucosylation, haptoglobin, hepatocellular carcinoma.


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APA
Shang, S., Li, W., Qin, X., Zhang, S., Liu, Y. (2017). Aided Diagnosis of Hepatocellular Carcinoma Using Serum Fucosylated Haptoglobin Ratios. Journal of Cancer, 8(5), 887-893. https://doi.org/10.7150/jca.17747.

ACS
Shang, S.; Li, W.; Qin, X.; Zhang, S.; Liu, Y. Aided Diagnosis of Hepatocellular Carcinoma Using Serum Fucosylated Haptoglobin Ratios. J. Cancer 2017, 8 (5), 887-893. DOI: 10.7150/jca.17747.

NLM
Shang S, Li W, Qin X, Zhang S, Liu Y. Aided Diagnosis of Hepatocellular Carcinoma Using Serum Fucosylated Haptoglobin Ratios. J Cancer 2017; 8(5):887-893. doi:10.7150/jca.17747. https://www.jcancer.org/v08p0887.htm

CSE
Shang S, Li W, Qin X, Zhang S, Liu Y. 2017. Aided Diagnosis of Hepatocellular Carcinoma Using Serum Fucosylated Haptoglobin Ratios. J Cancer. 8(5):887-893.

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