J Cancer 2015; 6(1):48-53. doi:10.7150/jca.10521
Upregulation of Acetylcholinesterase Mediated by p53 Contributes to Cisplatin-Induced Apoptosis in Human Breast Cancer Cell
1. Ningbo Institute of Medical Sciences, Ningbo 315020, China
2. Clinical Oncology Laboratory, Changzhou Cancer Hospital of Soochow University, Changzhou 213002, China
3. The Hepatobiliary Surgery Centre, the Ningbo No. 2 Hospital, Ningbo 315010, China
*These authors contributed equally to this work.
Ye X, Zhang C, Chen Y, Zhou T. Upregulation of Acetylcholinesterase Mediated by p53 Contributes to Cisplatin-Induced Apoptosis in Human Breast Cancer Cell. J Cancer 2015; 6(1):48-53. doi:10.7150/jca.10521. Available from http://www.jcancer.org/v06p0048.htm
Background: The expression of acetylcholinesterase (AChE) could be induced during apoptosis in various cell types. And reduced AChE expression either by siRNA could prevent apoptosis. However, the detailed mechanisms underlying the AChE regulation are largely unknown in human breast cancer cell.
Material and methods: MCF-7 cells were cultured and treated by cisplatin in the absence or presence of p53 siRNA.
Results: In this study, the regulation of AChE expression during apoptosis induced by cisplatin, a current used anticancer drug, was investigated in human breast cancer cell line MCF-7. Exposure of MCF-7 cells to cisplatin resulted in apoptosis in a time- and concentration-dependent manner. Meanwhile, the upregulated AChE and p53 were also observed during apoptosis. Silencing interfering RNA directed against p53 blocked the expression of AChE.
Conclusion: Taken together, these results suggested that AChE expression could be upregulated by the activation of p53 during apoptosis induced by cisplatin in MCF-7 cells.
Keywords: acetylcholinesterase, apoptosis, p53, cisplatin, breast cancer.