Elevated Pressure Improves the Rate of Formalin Penetration while Preserving Tissue Morphology

Chesnick, Ingrid E.; Mason, Jeffrey T.; O'Leary, Timothy J.; Fowler, Carol B.

doi:10.7150/jca.1.178

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J Cancer 2010; 1:178-183. doi:10.7150/jca.1.178 This volume Cite

Short Research Communication

Elevated Pressure Improves the Rate of Formalin Penetration while Preserving Tissue Morphology

Ingrid E. Chesnick¹, Jeffrey T. Mason¹, Timothy J. O'Leary², Carol B. Fowler^1,2 ^✉

1. Department of Biophysics, Armed Forces Institute of Pathology, Rockville, Maryland, USA;
2. Biomedical Laboratory Research and Development Service, Veterans Health Administration, Washington, DC, USA

Citation:

Chesnick IE, Mason JT, O'Leary TJ, Fowler CB. Elevated Pressure Improves the Rate of Formalin Penetration while Preserving Tissue Morphology. J Cancer 2010; 1:178-183. doi:10.7150/jca.1.178. https://www.jcancer.org/v01p0178.htm

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Abstract

Formaldehyde fixation and paraffin-embedding remains the most widely used technique for processing cancer tissue specimens for pathologic examination, the study of tissue morphology, and archival preservation. However, formaldehyde penetration and fixation is a slow process, requiring a minimum of 15 hr for routine processing of pathology samples. Routinely fixed samples often have a well-fixed outer rim, with a poorly-fixed inner core of tissue. In this study, we show that the application of elevated pressure up to 15,000 psi improves the rate of formaldehyde fixation by approximately 5 to 7-fold while preserving the tissue morphology of porcine liver. The tissue also exhibited much more uniform formaldehyde penetration after 30-60 min incubation under elevated pressure than samples fixed for the same length of time at atmospheric pressure.

Keywords: FFPE, fixation rate, formalin-fixed paraffin-embedded, high-pressure, histology

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APA

Chesnick, I.E., Mason, J.T., O'Leary, T.J., Fowler, C.B. (2010). Elevated Pressure Improves the Rate of Formalin Penetration while Preserving Tissue Morphology. Journal of Cancer, 1, 178-183. https://doi.org/10.7150/jca.1.178.

ACS

Chesnick, I.E.; Mason, J.T.; O'Leary, T.J.; Fowler, C.B. Elevated Pressure Improves the Rate of Formalin Penetration while Preserving Tissue Morphology. J. Cancer 2010, 1, 178-183. DOI: 10.7150/jca.1.178.

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CSE

Chesnick IE, Mason JT, O'Leary TJ, Fowler CB. 2010. Elevated Pressure Improves the Rate of Formalin Penetration while Preserving Tissue Morphology. J Cancer. 1:178-183.

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