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J Cancer 2023; 14(12):2386-2398. doi:10.7150/jca.83424 This issue Cite

Research Paper

Genome-wide RNA-sequencing dataset reveals AC096751.1 sever as a novel prognostic long non-coding RNA and its potential molecular mechanisms in patients with colon adenocarcinoma

Cun Liao1,2*, Zhiwen Gu1*, Wei Huang1,2, Yizhen Gong3, Xiwen Liao2,4, Minglin Lin1,2, Sen Zhang1,2✉

1. Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, People's Republic of China.
2. Guangxi Key Laboratory of Enhanced Recovery after Surgery for Gastrointestinal Cancer, Nanning, 530021, Guangxi Zhuang Autonomous Region, People's Republic of China.
3. Department of Clinical Research, Guangxi Medical University Cancer Hospital, 530021, Guangxi Zhuang Autonomous Region, People's Republic of China.
4. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, People's Republic of China.
*These authors have contributed equally to this work.

✉ Corresponding author: Prof. Sen Zhang, Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Guangxi Key Laboratory of Enhanced Recovery after Surgery for Gastrointestinal Cancer, Shuang Yong Road 6, More

Citation:
Liao C, Gu Z, Huang W, Gong Y, Liao X, Lin M, Zhang S. Genome-wide RNA-sequencing dataset reveals AC096751.1 sever as a novel prognostic long non-coding RNA and its potential molecular mechanisms in patients with colon adenocarcinoma. J Cancer 2023; 14(12):2386-2398. doi:10.7150/jca.83424. https://www.jcancer.org/v14p2386.htm
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Abstract

Graphic abstract

Objective: Through data analysis, we observed that AC096751.1 is markedly imbalance between colon adenocarcinoma (COAD) cancer and paracancerous tissues. However, the prognostic value and potential molecular mechanism of AC096751.1 in COAD are still unclear.

Methods: Whole genome RNA-sequencing datasets of The Cancer Genome Atlas (TCGA) COAD cohort were collected into current study, comprehensive survival analysis and bioinformatics function enrichment analysis approaches were apply to explore the clinical outcome and molecular mechanisms of AC096751.1 in COAD.

Results: In current study, we found that AC096751.1 is markedly down-regulated in COAD cancer tissues (log2 fold change =2.303, P<0.0001, false discovery rate <0.0001), and can be serve as a biomarker to distinguish COAD cancer and paracancerous tissues [area under curve=0.9518, 95% confidence interval (CI)=0.9261-0.9776]. Survival analysis suggests that low expression of AC096751.1 is connected with poor clinical outcome of COAD, and can serve as a novel prognostic indicator (log-rank P=0.016, adjusted P=0.005, hazard ratio=0.548, 95% CI=0.360-0.836). Bioinformatics function enrichment analysis suggests that the molecular mechanism of AC096751.1 in COAD may include participation in cell adhesion, cell proliferation, mitogen-activated protein kinase kinase (MAPKK), MAPK, janus-activated kinase-singal transducers and activators of transcriprion cascade, Erk1 and Erk 2 cascade, and nuclear factor-kappa B pathway. Tumor microenvironment and immune infiltration analysis indicates that COAD patients with different AC096751.1 expression have significant variation in tumor immune background.

Conclusion: The present study found that AC096751.1 is significantly differentially expressed in COAD and can be serve as a novel prognostic biomarker.

Keywords: long non-coding RNA, AC096751.1, colon adenocarcinoma, overall survival, The Cancer Genome Atlas.

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APA
Liao, C., Gu, Z., Huang, W., Gong, Y., Liao, X., Lin, M., Zhang, S. (2023). Genome-wide RNA-sequencing dataset reveals AC096751.1 sever as a novel prognostic long non-coding RNA and its potential molecular mechanisms in patients with colon adenocarcinoma. Journal of Cancer, 14(12), 2386-2398. https://doi.org/10.7150/jca.83424.

ACS
Liao, C.; Gu, Z.; Huang, W.; Gong, Y.; Liao, X.; Lin, M.; Zhang, S. Genome-wide RNA-sequencing dataset reveals AC096751.1 sever as a novel prognostic long non-coding RNA and its potential molecular mechanisms in patients with colon adenocarcinoma. J. Cancer 2023, 14 (12), 2386-2398. DOI: 10.7150/jca.83424.

NLM
Liao C, Gu Z, Huang W, Gong Y, Liao X, Lin M, Zhang S. Genome-wide RNA-sequencing dataset reveals AC096751.1 sever as a novel prognostic long non-coding RNA and its potential molecular mechanisms in patients with colon adenocarcinoma. J Cancer 2023; 14(12):2386-2398. doi:10.7150/jca.83424. https://www.jcancer.org/v14p2386.htm

CSE
Liao C, Gu Z, Huang W, Gong Y, Liao X, Lin M, Zhang S. 2023. Genome-wide RNA-sequencing dataset reveals AC096751.1 sever as a novel prognostic long non-coding RNA and its potential molecular mechanisms in patients with colon adenocarcinoma. J Cancer. 14(12):2386-2398.

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