J Cancer 2020; 11(18):5329-5344. doi:10.7150/jca.45394

Research Paper

Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma

Qinli Zhao1,2,3#, Xiwang Zheng1,2#, Huina Guo1,2, Xuting Xue1,2, Yuliang Zhang1,2, Min Niu1,2, Jiajia Cui1,2, Hongliang Liu1,2, Hongjie Luo1, Dongli Yang1,3, Yong Shi1,3, Hui Huangfu3,2, Shuxin Wen4,2, Yongyan Wu1,2,5,6✉, Wei Gao1,2,5✉, Binquan Wang1,2,3✉

1. Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.
2. Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.
3. Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.
4. Department of Otolaryngology Head & Neck Surgery, General Hospital of Shenzhen University, Shenzhen 518061, Guangdong, P. R. China.
5. Department of Biochemistry & Molecular Biology, Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.
6. Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, 030001 Shanxi, P. R. China.
#These authors contributed equally to this work.

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Citation:
Zhao Q, Zheng X, Guo H, Xue X, Zhang Y, Niu M, Cui J, Liu H, Luo H, Yang D, Shi Y, Huangfu H, Wen S, Wu Y, Gao W, Wang B. Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma. J Cancer 2020; 11(18):5329-5344. doi:10.7150/jca.45394. Available from https://www.jcancer.org/v11p5329.htm

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Abstract

At present, no blood-based biomarkers have been used in clinical practice for laryngeal squamous cell carcinoma (LSCC). Increasing evidence suggests that circulating exosomal microRNAs (miRNAs) may serve as potential diagnostic biomarkers for various cancers. This study aims to identify and evaluate serum exosomal miRNAs for LSCC diagnosis. The ExoQuick solution (EQ), which provides a high-yield and is a highly efficient exosome isolation method, was selected to isolate serum exosomes in the current study. In LSCC samples, exosome concentrations were higher than in healthy control (HC) samples. RNA-seq analysis identified a total of 1608 miRNAs, with 34 upregulated and 41 downregulated in LSCC samples relative to HC samples. Furthermore, qRT-PCR showed that miR-941 is significantly upregulated in LSCC serum exosomes, with this same trend seen in LSCC tissues and cells. Moreover, when examining miR-941 in cell lines, miR-941 overexpression promoted proliferation and invasion, while miR-941 knockdown inhibited cell proliferation and invasion. ROC curve analysis showed that miR-941 has an area under the curve (AUC) of 0.797 (95% CI = 0.676-0.918) for distinguishing LSCC patients from HCs. In conclusion, serum exosomal miR-941 may serve as a promising oncogenic biomarker for diagnosing LSCC, and has the potential as a therapeutic target.

Keywords: laryngeal squamous cell carcinoma, exosome, biomarker, hsa-miR-941, diagnosis