J Cancer 2020; 11(5):1240-1249. doi:10.7150/jca.38286

Research Paper

TRIM67 Promotes the Proliferation, Migration, and Invasion of Non-Small-Cell Lung Cancer by Positively Regulating the Notch Pathway

Jun Jiang1, Hongjiu Ren1, Yitong Xu1, Muli Wudu1, Qiongzi Wang1, Zongang Liu2, Hongbo Su1, Xizi Jiang1, Yao Zhang1, Bo Zhang1, Xueshan Qiu1✉

1. Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, China.
2. Department of Thoracic Surgery, Shengjing Hospital, China Medical University, No. 36 Sanhao St., Heping District, Shenyang, China.

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Citation:
Jiang J, Ren H, Xu Y, Wudu M, Wang Q, Liu Z, Su H, Jiang X, Zhang Y, Zhang B, Qiu X. TRIM67 Promotes the Proliferation, Migration, and Invasion of Non-Small-Cell Lung Cancer by Positively Regulating the Notch Pathway. J Cancer 2020; 11(5):1240-1249. doi:10.7150/jca.38286. Available from http://www.jcancer.org/v11p1240.htm

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Abstract

Tripartite motif-containing 67 (TRIM67), an E3 ubiquitin ligase, belongs to the TRIM protein family. The relationship between TRIM67 and tumorigenesis is not fully clear. Here, we elucidated TRIM67 function in non-small cell lung cancer (NSCLC). TRIM67 immunostaining results were correlated with clinicopathological features. Moreover, the function of TRIM67 in cultured NSCLC cells was evaluated by MTT, colony formation, and Transwell assays. TRIM67 expression was associated with tumor size, lymph node metastasis, p-TNM stage, cancer cell differentiation, and poor prognosis. We altered TRIM67 expression in A549 and H1299 cell lines, and the results showed that TRIM67 promoted cell proliferation, migration, invasion and EMT by positively regulating the Notch pathway. Collectively, the results showed that TRIM67 promotes NSCLC progression through the Notch pathway and that TRIM67 expression is associated with clinicopathological features, indicating that TRIM67 may play an important role in promoting the development of NSCLC and could be applied as not only an important prognostic biomarker but also a therapeutic target in NSCLC.

Keywords: non-small-cell lung cancer, TRIM67, cell proliferation, cell migration, cell invasion, Notch pathway