J Cancer 2020; 11(4):858-866. doi:10.7150/jca.35410

Research Paper

MiR-129-5p sensitization of lung cancer cells to etoposide-induced apoptosis by reducing YWHAB

Chengshan Xu1✉, Zhongli Du1, Simei Ren1, Xiaoshuan Liang2, Huihui Li3✉

1. National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology; Institute of GeriatricMedicine, Chinese Academy of Medical Sciences, Beijing, China
2. Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, China
3. Department of Medical Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong Province, China

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Citation:
Xu C, Du Z, Ren S, Liang X, Li H. MiR-129-5p sensitization of lung cancer cells to etoposide-induced apoptosis by reducing YWHAB. J Cancer 2020; 11(4):858-866. doi:10.7150/jca.35410. Available from http://www.jcancer.org/v11p0858.htm

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Abstract

Background: Lung cancer is the most common cause of death from cancer worldwide and recent studies have revealed that microRNAs play critical roles to regulate lung carcinogenesis. microRNA-129-5p (miR-129-5p) has been reported to regulate cell proliferation and invasion in lung cancer, but its role in lung cancer apoptosis remains unknown.

Methods: The expression of miR-129-5p and YWHAB in lung cancer tissues were analyzed from data downloaded from the NCBI Gene Expression Omnibus (GEO) database. Luciferase reporter assay, Western blot and qRT-PCR were used to determine the regulatory effect of miR-129-5p on YWHAB. Cell apoptosis was detected by using the PI/Annexin V Cell Apoptosis Kit. The effect of miR-129-5p and YWHAB on the survival of lung cancer patients was also explored.

Results: In this study, by combining the data derived from six GEO database, our results showed that miR-129-5p was downregulated in lung cancer tissues and YWHAB was upregulated in lung cancer patient' serum. A significant negative correlation between miR-129-5p and YWHAB was found in lung cancer tissues. Both the expression of YWHAB and miR-129-5p were associated significantly with prognosis (overall survival) in patients with lung cancer. Overexpression of miR-129-5p promotes VP16-induced lung cancer cell apoptosis and YWHAB was shown to be a direct downstream target of miR-129-5p.

Conclusion: Overexpression of expression miR-129-5p contributes to etoposide-induced lung cancer apoptosis by modulating YWHAB.

Keywords: Lung cancer, miR-129-5p, YWHAB, Apoptosis