Treatment of cancer cells with chemotherapeutic drugs results in profound changes in expression of genes encoding aldehyde-metabolizing enzymes

Zinovieva, Olga L.; Grineva, Evgeniya N.; Krasnov, George S.; Karpov, Dmitry S.; Zheltukhin, Andrei O.; Snezhkina, Anastasiya V.; Kudryavtseva, Anna V.; Mashkova, Tamara D.; Lisitsyn, Nikolai A.

doi:10.7150/jca.32608

International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

open access Global reach, higher impact

Full Text | PDF

J Cancer 2019; 10(18):4256-4263. doi:10.7150/jca.32608 This issue Cite

Research Paper

Treatment of cancer cells with chemotherapeutic drugs results in profound changes in expression of genes encoding aldehyde-metabolizing enzymes

Olga L. Zinovieva¹, Evgeniya N. Grineva¹, George S. Krasnov^1,2, Dmitry S. Karpov^1,2, Andrei O. Zheltukhin¹, Anastasiya V. Snezhkina¹, Anna V. Kudryavtseva¹, Tamara D. Mashkova¹, Nikolai A. Lisitsyn^1✉

1. Engelhardt Institute of Molecular Biology, the Russian Academy of Sciences, 119991 Moscow, Russia
2. Institute of Biomedical Chemistry, the Russian Academy of Sciences, 119121 Moscow, Russia

Citation:

Zinovieva OL, Grineva EN, Krasnov GS, Karpov DS, Zheltukhin AO, Snezhkina AV, Kudryavtseva AV, Mashkova TD, Lisitsyn NA. Treatment of cancer cells with chemotherapeutic drugs results in profound changes in expression of genes encoding aldehyde-metabolizing enzymes. J Cancer 2019; 10(18):4256-4263. doi:10.7150/jca.32608. https://www.jcancer.org/v10p4256.htm

Other styles

Abstract

Using RNA-seq, RT-qPCR, and bioinformatics we have studied the influence of a wide spectrum of chemotherapeutic drugs on transcription of AKR1B10, AKR1C1, ALDH1A1, and ALDH1A3 genes, which encode the major aldehyde-metabolizing enzymes. The strongest alterations were detected in case of AKR1B10 mRNA that was significantly upregulated in wild type p53 cancer cells, but downregulated in mutant p53 cancer cells. Subsequent experiments demonstrated the significant and consistent decrease in the AKR1B10 mRNA content in sera of colon cancer patients, as compared to sera of healthy donors (p<0.0001, SPE=92.9%, SNE=79.3%, AUC=0.889), which implies that this RNA is a valuable marker for serological diagnosis of colorectal cancer. Moreover, we have found that ALDH1A3 protein is a key inactivator of ROS-generated aldehydes, which is a perspective target for the development of new chemotherapeutic drugs.

Keywords: colorectal cancer, chemotherapeutic drugs, reactive oxygen species, RNA-seq, serum biomarkers.

Citation styles

APA

Zinovieva, O.L., Grineva, E.N., Krasnov, G.S., Karpov, D.S., Zheltukhin, A.O., Snezhkina, A.V., Kudryavtseva, A.V., Mashkova, T.D., Lisitsyn, N.A. (2019). Treatment of cancer cells with chemotherapeutic drugs results in profound changes in expression of genes encoding aldehyde-metabolizing enzymes. Journal of Cancer, 10(18), 4256-4263. https://doi.org/10.7150/jca.32608.

ACS

Zinovieva, O.L.; Grineva, E.N.; Krasnov, G.S.; Karpov, D.S.; Zheltukhin, A.O.; Snezhkina, A.V.; Kudryavtseva, A.V.; Mashkova, T.D.; Lisitsyn, N.A. Treatment of cancer cells with chemotherapeutic drugs results in profound changes in expression of genes encoding aldehyde-metabolizing enzymes. J. Cancer 2019, 10 (18), 4256-4263. DOI: 10.7150/jca.32608.

NLM

CSE

Zinovieva OL, Grineva EN, Krasnov GS, Karpov DS, Zheltukhin AO, Snezhkina AV, Kudryavtseva AV, Mashkova TD, Lisitsyn NA. 2019. Treatment of cancer cells with chemotherapeutic drugs results in profound changes in expression of genes encoding aldehyde-metabolizing enzymes. J Cancer. 10(18):4256-4263.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.