J Cancer 2017; 8(17):3456-3463. doi:10.7150/jca.19345

Research Paper

Identification of circular RNA signature in bladder cancer

Xiao Yang*, Wenbo Yuan*, Jun Tao*, Peng Li, Chengdi Yang, Xiaheng Deng, Xiaolei Zhang, Jingyuan Tang, Jie Han, Jingzi Wang, Pengchao Li, Qiang Lu, Min Gu

Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210006, China
* These authors contributed equally to this work.

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Yang X, Yuan W, Tao J, Li P, Yang C, Deng X, Zhang X, Tang J, Han J, Wang J, Li P, Lu Q, Gu M. Identification of circular RNA signature in bladder cancer. J Cancer 2017; 8(17):3456-3463. doi:10.7150/jca.19345. Available from http://www.jcancer.org/v08p3456.htm

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Circular RNA (circRNA) comprises a class of endogenous species of RNA consisting of a circular loop that is crucial for genetic and epigenetic regulation. The significance of circRNA in bladder cancer (BCa) remains to be investigated. Here we performed genome‑wide circRNA analysis of 5 paired tumour and adjacent normal tissue samples from BCa patients via next generation sequencing (NGS) technology. Next we confirmed NGS data in a separate set of 32 paired BCa samples using quantitative real-time reverse transcription polymerase chain reaction. The results showed that circRNA profile presented a total of 88,732 circRNA in BCa samples. Among them, 14 were upregulated and 42 were downregulated with q-values of <0.001 and fold changes of ≥2 or ≤0.5. The expression level changes of hsa_circ_0091017 and hsa_circ_0002024 in the 32 paired samples were in accord with NGS data. In conclusion, we identified a set of circRNAs that are potentially implicated in the tumorigenesis of BCa and could serve as novel diagnostic markers for BCa.

Keywords: bladder cancer, circular RNA, next generation sequencing