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J Cancer 2016; 7(1):42-49. doi:10.7150/jca.12923 This issue Cite

Research Paper

Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells

Aino N.E. Salmiheimo1, Harri K. Mustonen1, Sanna A.A. Vainionpää1, Zhanlong Shen2, Esko A.J. Kemppainen1, Hanna E. Seppänen1*, Pauli A. Puolakkainen1*✉

1. Department of Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland
2. Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing, China
*H. Seppänen and P. Puolakkainen share equal last authorship.

✉ Corresponding author: Pauli Antero Puolakkainen, MD, PhD, Professor of Surgery. Address: Department of Surgery, Helsinki University Central Hospital, Kasarmikatu 11-13, P.O. Box 263, 00130 Helsinki, FINLAND. Phone number: +358 50 582 9785; E-mail: p More

Citation:
Salmiheimo ANE, Mustonen HK, Vainionpää SAA, Shen Z, Kemppainen EAJ, Seppänen HE, Puolakkainen PA. Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells. J Cancer 2016; 7(1):42-49. doi:10.7150/jca.12923. https://www.jcancer.org/v07p0042.htm
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Abstract

Recent studies suggest that pro-inflammatory type M1 macrophages inhibit tumor progression and that anti-inflammatory M2 macrophages enhance it. The aim of this study was to examine the interaction of type M1 and M2 macrophages with pancreatic cancer cells. We studied the migration rate of fluorescein stained pancreatic cancer cells on Matrigel cultured alone or with Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) differentiated macrophages or with Macrophage Colony Stimulating Factor (M-CSF) differentiated macrophages, skewing the phenotype towards pro- and anti-inflammatory direction, respectively. Macrophage differentiation was assessed with flow cytometry and the cytokine secretion in cell cultures with cytokine array. Both GM-CSF and M-CSF differentiated macrophages increased the migration rate of primary pancreatic adenocarcinoma cell line (MiaPaCa-2) and metastatic cell line (HPAF-II). Stimulation with IL6 or IL4+LPS reversed the macrophages' increasing effect on the migration rate of MiaPaCa-2 completely and partly of HPAF-II. Co-culture with MiaPaCa-2 reduced the inflammatory cytokine secretion of GM-CSF differentiated macrophages. Co-culture of macrophages with pancreatic cancer cells seem to change the inflammatory cytokine profile of GM-CSF differentiated macrophages and this might explain why also GM-CSF differentiated macrophages promoted the invasion. Adding IL6 or IL4+LPS to the cell culture with MiaPaCa-2 and GM-CSF or M-CSF differentiated macrophages increased the secretion of inflammatory cytokines and this could contribute to the reversion of the macrophage induced increase of cancer cell migration rate.

Keywords: pancreatic adenocarcinoma, M1, M2, macrophages, invasion

Citation styles

APA
Salmiheimo, A.N.E., Mustonen, H.K., Vainionpää, S.A.A., Shen, Z., Kemppainen, E.A.J., Seppänen, H.E., Puolakkainen, P.A. (2016). Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells. Journal of Cancer, 7(1), 42-49. https://doi.org/10.7150/jca.12923.

ACS
Salmiheimo, A.N.E.; Mustonen, H.K.; Vainionpää, S.A.A.; Shen, Z.; Kemppainen, E.A.J.; Seppänen, H.E.; Puolakkainen, P.A. Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells. J. Cancer 2016, 7 (1), 42-49. DOI: 10.7150/jca.12923.

NLM
Salmiheimo ANE, Mustonen HK, Vainionpää SAA, Shen Z, Kemppainen EAJ, Seppänen HE, Puolakkainen PA. Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells. J Cancer 2016; 7(1):42-49. doi:10.7150/jca.12923. https://www.jcancer.org/v07p0042.htm

CSE
Salmiheimo ANE, Mustonen HK, Vainionpää SAA, Shen Z, Kemppainen EAJ, Seppänen HE, Puolakkainen PA. 2016. Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells. J Cancer. 7(1):42-49.

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