J Cancer
2015; 6(3):227-232.
doi:10.7150/jca.10765 This issueCite
Short Research Communication
MicroRNA-210 and Endoplasmic Reticulum Chaperones in the Regulation of Chemoresistance in Glioblastoma
Derek Lee, Stella Sun, Xiao Qin Zhang, Ping De Zhang, Amy S.W. Ho, Karrie M.Y. Kiang, Ching Fai Fung, Wai Man Lui, Gilberto K.K. Leung✉
Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong
✉ Corresponding author: Dr. Gilberto K.K. Leung, Clinical Associate Professor, Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong. Tel: +852 2255 3368, Fax: +852 2818 4350, E-mail: gilbertohkMore
Citation:
Lee D, Sun S, Zhang XQ, Zhang PD, Ho ASW, Kiang KMY, Fung CF, Lui WM, Leung GKK. MicroRNA-210 and Endoplasmic Reticulum Chaperones in the Regulation of Chemoresistance in Glioblastoma. J Cancer 2015; 6(3):227-232. doi:10.7150/jca.10765. https://www.jcancer.org/v06p0227.htm
Glioblastoma multiforme (GBM) is the commonest primary brain tumour in adults characterized by relentless recurrence due to resistance towards the standard chemotherapeutic agent temozolomide (TMZ). Prolyl 4-hydroxylase, beta polypeptide (P4HB), an endoplasmic reticulum (ER) chaperone, is known to be upregulated in TMZ-resistant GBM cells. MicroRNAs (miRNAs) are non-protein-coding transcripts that may play important roles in GBM chemoresistance. We surmised that miRNA dysregulations may contribute to P4HB upregulation, hence chemoresistance. We found that miRNA-210 (miR-210) was P4HB-targeting and was highly downregulated in TMZ-resistant GBM cells. Forced overexpression of miR-210 led to P4HB downregulation and a reduction in TMZ-resistance. A reciprocal relationship between their expressions was also verified in clinical glioma specimens. Our study is the first to demonstrate a potential link between miR-210 and ER chaperone in determining chemosensitivity in GBM. The findings have important translational implications in suggesting new directions of future studies.
Keywords: Glioblastoma, miRNA, P4HB, ER stress reponse, chemoresistance, temozolomide
Citation styles
APA
Lee, D., Sun, S., Zhang, X.Q., Zhang, P.D., Ho, A.S.W., Kiang, K.M.Y., Fung, C.F., Lui, W.M., Leung, G.K.K. (2015). MicroRNA-210 and Endoplasmic Reticulum Chaperones in the Regulation of Chemoresistance in Glioblastoma. Journal of Cancer, 6(3), 227-232. https://doi.org/10.7150/jca.10765.
ACS
Lee, D.; Sun, S.; Zhang, X.Q.; Zhang, P.D.; Ho, A.S.W.; Kiang, K.M.Y.; Fung, C.F.; Lui, W.M.; Leung, G.K.K. MicroRNA-210 and Endoplasmic Reticulum Chaperones in the Regulation of Chemoresistance in Glioblastoma. J. Cancer 2015, 6 (3), 227-232. DOI: 10.7150/jca.10765.
NLM
Lee D, Sun S, Zhang XQ, Zhang PD, Ho ASW, Kiang KMY, Fung CF, Lui WM, Leung GKK. MicroRNA-210 and Endoplasmic Reticulum Chaperones in the Regulation of Chemoresistance in Glioblastoma. J Cancer 2015; 6(3):227-232. doi:10.7150/jca.10765. https://www.jcancer.org/v06p0227.htm
CSE
Lee D, Sun S, Zhang XQ, Zhang PD, Ho ASW, Kiang KMY, Fung CF, Lui WM, Leung GKK. 2015. MicroRNA-210 and Endoplasmic Reticulum Chaperones in the Regulation of Chemoresistance in Glioblastoma. J Cancer. 6(3):227-232.