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J Cancer 2022; 13(1):102-111. doi:10.7150/jca.61961 This issue Cite

Research Paper

LncRNA PCAT19 induced by SP1 and acted as oncogene in gastric cancer competitively binding to miR429 and upregulating DHX9

Lei Xiao1, Weijie Yuan1, Changhao Huang1, Qingqing Luo2, Runsha Xiao1, Zi-Hua Chen1✉

1. Department of Gastrointestinal, Xiangya Hospital, Central South University, Changsha 410008, China.
2. Department of Oncology, Hunan Provincial People's Hospital, Changsha 410002, China.

✉ Corresponding author: Department of General Surgery, Xiangya Hospital of Central South University, Xiangya Road 87, Kaifu District, Changsha 410008, Hunan Province, China. Tel.: +8613808413012. E-mail: zihuaccom

Citation:
Xiao L, Yuan W, Huang C, Luo Q, Xiao R, Chen ZH. LncRNA PCAT19 induced by SP1 and acted as oncogene in gastric cancer competitively binding to miR429 and upregulating DHX9. J Cancer 2022; 13(1):102-111. doi:10.7150/jca.61961. https://www.jcancer.org/v13p0102.htm
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Abstract

Graphic abstract

Increasing evidence suggests that long non-coding RNAs (lncRNAs) are crucial in cancer biological processes. To investigate if lncRNA contributes to gastric cancer (GC), we conducted a bioinformatics analysis in human microarray datasets, and the results showed that lncRNA prostate cancer-associated transcript 19 (PCAT19) was upregulated in GC. Quantitative reverse-transcriptase PCR and in situ hybridization assays also revealed that PCAT19 was upregulated in GC tissues. The PCAT19 expression in GC was significantly related to tumor size, lymph node metastasis, and pathological stage. Moreover, patients with higher PCAT19 expression levels were more likely to have a poor prognosis for overall survival. The knockdown of PCAT19 by siRNA significantly suppressed the proliferation and invasion of GC cells. The cell distribution of PCAT19 in GC cells was examined by fluorescence in situ hybridization assay, and the results showed that it was mainly located in the cytoplasm. Mechanistically, PCAT19 sponges miR-429 and promotes DHX9 expression. In addition, the transcription factor SP1 is involved in PCAT19 activation. Our results demonstrate that lncRNA PCAT19 is induced by SP1 and acts as an oncogene in GC that competitively binds to miR429 and upregulates DHX9.

Keywords: LncRNA, PCAT19, Gastric cancer, SP1, miR-429, DHX9

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APA
Xiao, L., Yuan, W., Huang, C., Luo, Q., Xiao, R., Chen, Z.H. (2022). LncRNA PCAT19 induced by SP1 and acted as oncogene in gastric cancer competitively binding to miR429 and upregulating DHX9. Journal of Cancer, 13(1), 102-111. https://doi.org/10.7150/jca.61961.

ACS
Xiao, L.; Yuan, W.; Huang, C.; Luo, Q.; Xiao, R.; Chen, Z.H. LncRNA PCAT19 induced by SP1 and acted as oncogene in gastric cancer competitively binding to miR429 and upregulating DHX9. J. Cancer 2022, 13 (1), 102-111. DOI: 10.7150/jca.61961.

NLM
Xiao L, Yuan W, Huang C, Luo Q, Xiao R, Chen ZH. LncRNA PCAT19 induced by SP1 and acted as oncogene in gastric cancer competitively binding to miR429 and upregulating DHX9. J Cancer 2022; 13(1):102-111. doi:10.7150/jca.61961. https://www.jcancer.org/v13p0102.htm

CSE
Xiao L, Yuan W, Huang C, Luo Q, Xiao R, Chen ZH. 2022. LncRNA PCAT19 induced by SP1 and acted as oncogene in gastric cancer competitively binding to miR429 and upregulating DHX9. J Cancer. 13(1):102-111.

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