J Cancer 2020; 11(9):2572-2579. doi:10.7150/jca.37959

Research Paper

Upregulation of LncRNA PVT1 Facilitates Pancreatic Ductal Adenocarcinoma Cell Progression and Glycolysis by Regulating MiR-519d-3p and HIF-1A

Junwei Sun1*, Pingping Zhang2*, Tao Yin3, Feng Zhang3, Weixing Wang1✉

1. Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan, Hubei 430060, China.
2. Department of Radiation Oncology, Hubei Cancer Hospital, Affiliated Hubei Cancer Hospital of Huazhong University of Science and Technology, 116 Zhuodaoquan South Road, Wuhan, Hubei 430079, China.
3. Department of Hepatic & Biliary & Pancreatic Surgery, Hubei Cancer Hospital, Affiliated Hubei Cancer Hospital of Huazhong University of Science and Technology, 116 Zhuodaoquan South Road, Wuhan, Hubei 430079, China.
*Junwei Sun and Pingping Zhang contributed equally to this work.

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Citation:
Sun J, Zhang P, Yin T, Zhang F, Wang W. Upregulation of LncRNA PVT1 Facilitates Pancreatic Ductal Adenocarcinoma Cell Progression and Glycolysis by Regulating MiR-519d-3p and HIF-1A. J Cancer 2020; 11(9):2572-2579. doi:10.7150/jca.37959. Available from http://www.jcancer.org/v11p2572.htm

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Abstract

The long, noncoding RNA (lncRNA) PVT1, as an important epigenetic regulator, has a critical role in carcinogenesis. However, its role in pancreatic ductal adenocarcinoma (PDAC) has not been fully investigated. Here, the up-regulated expression of lncRNA PVT1 is found in our PDAC tumor samples. Knockdown of it suppressed PDCA cells growth and glycolysis. An inverse association between miR-519d-3p and PVT1 was found. RIP, RNA pulldown and luciferase assay showed that PVT1 directly targets miR-519d-3p by binding with microRNA binding site. Bioinformatics analysis and study indicated that HIF-1A is a target of miR-519d-3p. Collectively, our findings suggested that PVT1 could act as an oncogenic lncRNA, and promote tumor progression by regulating HIF-1A via competing with miR-519d-3p.

Keywords: PDAC, PVT1, miR-519d-3p, HIF-1A