J Cancer 2019; 10(12):2594-2600. doi:10.7150/jca.33192

Research Paper

Involvement of endothelial nitric oxide synthase gene variants in the aggressiveness of uterine cervical cancer

Wei-Chen Hung1,#, Ting-Feng Wu2,3,#, Soo-Cheen Ng2,3, Yueh‐Chun Lee1,3,4, Huang-Pin Shen1,2,3, Shun-Fa Yang1,5, Po-Hui Wang 1,2,3,✉

1. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan
2. Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan
3. School of Medicine, Chung Shan Medical University, Taichung, Taiwan
4. Radiation Oncology Department, Chung Shan Medical University Hospital, Taichung, Taiwan
5. Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan
#These authors contributed equally to this work.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Citation:
Hung WC, Wu TF, Ng SC, Lee Y, Shen HP, Yang SF, Wang PH. Involvement of endothelial nitric oxide synthase gene variants in the aggressiveness of uterine cervical cancer. J Cancer 2019; 10(12):2594-2600. doi:10.7150/jca.33192. Available from http://www.jcancer.org/v10p2594.htm

File import instruction

Abstract

To date, few studies explore the involvement of endothelial nitric oxide synthase (eNOS) gene variants in uterine cervical cancer. Therefore, we conducted this study to assess the clinical implication of eNOS in cervical carcinogenesis, clinicopathological characteristics and patient survival. One hundred and seventeen patients with cervical invasive cancer and 95 with preinvasive lesions and 330 control women were consecutively enrolled. Real time polymerase chain reaction was used to examine the genotypic distributions of eNOS single nucleotide polymorphisms (SNPs) rs1799983 (894G>T) at the exon 7 region and rs2070744 (-786T>C) at the promoter region. Our results indicated no significant associations among genotypic distributions of eNOS SNPs and patients with cervical invasive cancer and those with preinvasive lesions as well as normal controls. However, cervical cancer patients with genotypes TC/CC in eNOS SNP rs2070744 carried less risk of advanced stage [odds ratios (OR) = 0.30, 95% confidence interval (CI)=0.09-0.97, p=0.036], parametrium invasion (OR=0.16, 95% CI=0.02-0.75, p=0.009) and pelvic lymph node metastasis (OR=0.12, 95% CI=0.01-0.89, p=0.016). In conclusion, although eNOS SNPs rs2070744 and rs1799983 do not display significant associations with cervical carcinogenesis and patient survival, cervical cancer patients with genotypes TC/CC in rs2070744 carry less risk of advanced stage, parametrium invasion and pelvic lymph node metastasis in Taiwan.

Keywords: endothelial nitric oxide synthase gene, single nucleotide polymorphism, uterine cervical cancer, parametrium invasion, pelvic lymph node metastasis