Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway

Tang, Guiliang; Zeng, Zihang; Sun, Wenjie; Li, Shuying; You, Chengcheng; Tang, Fang; Peng, Shan; Ma, Shijing; Luo, Yuan; Xu, Jieyu; Tian, Xiaoli; Zhang, Nannan; Gong, Yan; Xie, Conghua

doi:10.7150/jca.31077



International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

Global reach, higher impact

Full Text | PDF

J Cancer 2019; 10(10):2261-2275. doi:10.7150/jca.31077 This issue Cite

Research Paper

Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway

Guiliang Tang¹, Zihang Zeng¹, Wenjie Sun¹, Shuying Li¹, Chengcheng You¹, Fang Tang¹, Shan Peng¹, Shijing Ma¹, Yuan Luo¹, Jieyu Xu¹, Xiaoli Tian¹, Nannan Zhang¹, Yan Gong^2✉, Conghua Xie^1,3,4✉

1. Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China
2. Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China
3. Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China
4. Hubei Cancer Clinical Study Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China

Citation:

Tang G, Zeng Z, Sun W, Li S, You C, Tang F, Peng S, Ma S, Luo Y, Xu J, Tian X, Zhang N, Gong Y, Xie C. Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway. J Cancer 2019; 10(10):2261-2275. doi:10.7150/jca.31077. https://www.jcancer.org/v10p2261.htm

Other styles

Abstract

Objective: Increasing evidence suggested that dysregulated small nucleolar RNAs (snoRNAs) were involved in tumor development. The roles of snoRNA 71A (SNORA71A) in the progression of non-small cell lung cancer (NSCLC) remained unclear.

Methods: Dataset GSE19188 from Gene Expression Omnibus (GEO) database was downloaded to detect the expression levels of SNORA71A in NSCLC tissues. The biological significance of SNORA71A was explored by loss-of-function analysis both in vitro and in vivo.

Results: SNORA71A was overexpressed in NSCLC tissues compared with normal tissues, and upregulated SNORA71A was significantly associated with worse survival of NSCLC patients. Knockdown of SNORA71A suppressed proliferation of both A549 and PC9 cells, and induced G0/G1 phase arrest. Knockdown of SNORA71A also suppressed xenograft tumor growth in mice. In addition, knockdown of SNORA71A inhibited cell invasion and migration and suppressed epithelial-mesenchymal transition. Furthermore, downregulated SNORA71A decreased the phosphorylation of MEK and ERK1/2 in the MAPK/ERK signal pathway.

Conclusion: SNORA71A functions as an oncogene in NSCLC and may serve as a therapeutic target and promising prognostic biomarker of NSCLC.

Keywords: Non-small cell lung cancer, Small nucleolar RNA, SNORA71A, MAPK/ERK, Tumorigenesis

Citation styles

APA

Tang, G., Zeng, Z., Sun, W., Li, S., You, C., Tang, F., Peng, S., Ma, S., Luo, Y., Xu, J., Tian, X., Zhang, N., Gong, Y., Xie, C. (2019). Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway. Journal of Cancer, 10(10), 2261-2275. https://doi.org/10.7150/jca.31077.

ACS

Tang, G.; Zeng, Z.; Sun, W.; Li, S.; You, C.; Tang, F.; Peng, S.; Ma, S.; Luo, Y.; Xu, J.; Tian, X.; Zhang, N.; Gong, Y.; Xie, C. Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway. J. Cancer 2019, 10 (10), 2261-2275. DOI: 10.7150/jca.31077.

NLM

CSE

Tang G, Zeng Z, Sun W, Li S, You C, Tang F, Peng S, Ma S, Luo Y, Xu J, Tian X, Zhang N, Gong Y, Xie C. 2019. Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway. J Cancer. 10(10):2261-2275.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.