J Cancer 2019; 10(5):1154-1161. doi:10.7150/jca.29052 This issue Cite

Research Paper

PD-L1 expression patterns in tumour cells and their association with CD8+ tumour infiltrating lymphocytes in clear cell renal cell carcinoma

Qian Zhu1,2, Mu-Yan Cai1,3, De-Sheng Weng1,2, Jing-Jing Zhao1,2, Qiu-Zhong Pan1,2, Qi-Jing Wang1,2, Yan Tang1,2, Jia He1,2, Min Li1,3, Jian-Chuan Xia1,2✉

1. Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, P. R. China
2. Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou 510060, People's Republic of China
3. Department of Pathology, Sun Yat-Sen University Cancer Center, Guangzhou 510060, People's Republic of China

Citation:
Zhu Q, Cai MY, Weng DS, Zhao JJ, Pan QZ, Wang QJ, Tang Y, He J, Li M, Xia JC. PD-L1 expression patterns in tumour cells and their association with CD8+ tumour infiltrating lymphocytes in clear cell renal cell carcinoma. J Cancer 2019; 10(5):1154-1161. doi:10.7150/jca.29052. https://www.jcancer.org/v10p1154.htm
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Abstract

Purpose: To evaluate the tumour cell PD-L1 (TC-PD-L1) expression patterns in the local microenvironment of clear cell renal cell carcinoma (ccRCC).

Materials and Methods: 30 fresh primary ccRCC tissues were used to detect the association between TC-PD-L1 and CD8+TILs at mRNA level. The in vitro incubation experiment was used to confirm the association between extrinsic TC-PD-L1 expression and IFNγ. A cohort of 135 ccRCC patients treated between January 2009 and August 2013 was included for survival analysis.

Results: Our results confirmed that ccRCC cell lines were capable of expressing PD-L1. The incubation experiment in vitro demonstrated the positive correlation of TC-PD-L1 expression with interferon-gamma (IFNγ). Additionally, survival analysis was investigated in 135 ccRCC patients and found no independent correlation of TC-PD-L1 expression in multivariate analysis, whereas more distinct prognostic differences were detected when TC-PD-L1-positive ccRCC were sub-classified as with or without CD8+ T cell infiltration.

Conclusion: The intrinsic and extrinsic expression patterns are both detected in ccRCC. High positive rate of TC-PD-L1 correlated closely to the strong infiltration of CD8+ TILs. TC-PD-L1-positive ccRCC patients with abundant CD8+ TILs infiltration confer the high risk of death and disease relapse.

Keywords: PD-L1, local microenvironment, intrinsic expression, extrinsic expression, clear cell renal cell carcinoma


Citation styles

APA
Zhu, Q., Cai, M.Y., Weng, D.S., Zhao, J.J., Pan, Q.Z., Wang, Q.J., Tang, Y., He, J., Li, M., Xia, J.C. (2019). PD-L1 expression patterns in tumour cells and their association with CD8+ tumour infiltrating lymphocytes in clear cell renal cell carcinoma. Journal of Cancer, 10(5), 1154-1161. https://doi.org/10.7150/jca.29052.

ACS
Zhu, Q.; Cai, M.Y.; Weng, D.S.; Zhao, J.J.; Pan, Q.Z.; Wang, Q.J.; Tang, Y.; He, J.; Li, M.; Xia, J.C. PD-L1 expression patterns in tumour cells and their association with CD8+ tumour infiltrating lymphocytes in clear cell renal cell carcinoma. J. Cancer 2019, 10 (5), 1154-1161. DOI: 10.7150/jca.29052.

NLM
Zhu Q, Cai MY, Weng DS, Zhao JJ, Pan QZ, Wang QJ, Tang Y, He J, Li M, Xia JC. PD-L1 expression patterns in tumour cells and their association with CD8+ tumour infiltrating lymphocytes in clear cell renal cell carcinoma. J Cancer 2019; 10(5):1154-1161. doi:10.7150/jca.29052. https://www.jcancer.org/v10p1154.htm

CSE
Zhu Q, Cai MY, Weng DS, Zhao JJ, Pan QZ, Wang QJ, Tang Y, He J, Li M, Xia JC. 2019. PD-L1 expression patterns in tumour cells and their association with CD8+ tumour infiltrating lymphocytes in clear cell renal cell carcinoma. J Cancer. 10(5):1154-1161.

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