Department of Gastroenterological Surgery, West China Hospital, Sichuan University, Chengdu, 610041
This study aimed to explore the mechanism of lung metastases of colorectal cancer. The expression of CXCR4, CXCR7, and CXCL12 in colorectal cancer and lung metastases was analyzed by the immunohistochemical (IHC) method. Further, the study aimed at getting new insights into the role of CXCL12 in lung metastasis. A total of 41 patients who underwent surgical resections for both colorectal cancer and lung metastasis were retrospectively selected for this study. Also, 41 patients who underwent lung resection for benign lung disease were selected as the control group. In the in vivo experiment, the mice were injected with exosomes isolated from CT26. The mice with and without injection were sacrificed 3 weeks later. The lung tissue was analyzed by the IHC method and quantitative real-time PCR. The expression of CXCR7 and CXCL12 was significantly higher in lung metastases than in paired primary lesions. The expression of CXCL12 was significantly higher in the noncancerous lung tissue of patients with CRC than in patients with benign lung disease. Also, the expression of CXCL12 in the lung was higher in mice with exosome education. The results inferred that the expression of CXCR7 and CXCL12 was significantly higher in lung metastases than in primary tumors. Their combination could promote colorectal cancer lung metastasis. The expression of CXCL12 was elevated before metastasis. And this effect was induced by exosomes.
Keywords: colorectal cancer, CXCL12, exosome, lung metastasis