Could the Anti-Chaperone VER155008 Replace Temozolomide for Glioma Treatment

Shervington, Leroy; Patil, Harshada; Shervington, Amal

doi:10.7150/jca.12251



International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

Global reach, higher impact

Full Text | PDF

J Cancer 2015; 6(8):786-794. doi:10.7150/jca.12251 This issue Cite

Research Paper

Could the Anti-Chaperone VER155008 Replace Temozolomide for Glioma Treatment

Leroy Shervington, Harshada Patil, Amal Shervington^✉

Brain Tumour North West, Faculty of Science and Technology, University of Central Lancashire, Preston, PR1 2HE. UK.

Citation:

Shervington L, Patil H, Shervington A. Could the Anti-Chaperone VER155008 Replace Temozolomide for Glioma Treatment. J Cancer 2015; 6(8):786-794. doi:10.7150/jca.12251. https://www.jcancer.org/v06p0786.htm

Other styles

Abstract

Cancer inducible molecular chaperone HSP90 is of great importance as an anticancer target. Proteomic analysis showed that inhibiting HSP90 by the geldanamycin derivative, 17-AAG elevated the expression of the co-chaperone Hsp70. In this study we used HSP90 selective inhibitor 17-AAG and HSP70/90 dual inhibitor, VER155008 (VER) in U87-MG glioma cells. miRNAs microarray technology was used to evaluate the efficacy of these inhibitory drugs compared with temozolomide (TMZ), used as a standard treatment for glioma. Microarrays data identified 154 differentially expressed miRNAs using stringent or unstringent parameters. 16 miRNAs were overlapped between treatments, 13 upregulated and one downregulated miRNA were overlapped between TMZ and VER. The miRNA target prediction software was used for these overlapped miRNAs and identified 6 of the 13 upregulated miRNAs target methyltransferase genes. The IC₅₀, together with Akt and HSP70 and 90 protein level data favour VER and TMZ to 17-AAG, however due to the selectivity of VER to cancer cells as a potent antichaperon, it may be more favourable to the standard TMZ.

Keywords: HSP70, HSP90, miRNA, glioma cells, 17-AAG, VER (VER155008 ), TMZ

Citation styles

APA

Shervington, L., Patil, H., Shervington, A. (2015). Could the Anti-Chaperone VER155008 Replace Temozolomide for Glioma Treatment. Journal of Cancer, 6(8), 786-794. https://doi.org/10.7150/jca.12251.

ACS

Shervington, L.; Patil, H.; Shervington, A. Could the Anti-Chaperone VER155008 Replace Temozolomide for Glioma Treatment. J. Cancer 2015, 6 (8), 786-794. DOI: 10.7150/jca.12251.

NLM

CSE

Shervington L, Patil H, Shervington A. 2015. Could the Anti-Chaperone VER155008 Replace Temozolomide for Glioma Treatment. J Cancer. 6(8):786-794.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.