Zhoushi Qiling decoction inhibits proliferation of human prostate cancer cells through IL6/STAT3 pathway

Cao, Hongwen; Feng, Yigeng; Sun, Peng; Chen, Lei; Wang, Dan; Gao, Renjie

doi:10.7150/jca.84943

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J Cancer 2023; 14(12):2246-2254. doi:10.7150/jca.84943 This issue Cite

Research Paper

Zhoushi Qiling decoction inhibits proliferation of human prostate cancer cells through IL6/STAT3 pathway

Hongwen Cao^#, Yigeng Feng^#, Peng Sun^#, Lei Chen^✉, Dan Wang^✉, Renjie Gao^✉

Surgical Department I (Urology Department), LONGHUA Hospital Shanghai University of Traditional Chinese Medicine, No. 725 Wanping Road South, Xuhui District, Shanghai 200032, China.
#These authors contributed equally to this work.

✉ Corresponding authors: Lei Chen; Surgical Department I (Urology Department), LONGHUA Hospital Shanghai University of Traditional Chinese Medicine, No. 725 Wanping Road South, Xuhui District, Shanghai 200032, China; Email: chenlei2114edu.cn; More

Citation:

Cao H, Feng Y, Sun P, Chen L, Wang D, Gao R. Zhoushi Qiling decoction inhibits proliferation of human prostate cancer cells through IL6/STAT3 pathway. J Cancer 2023; 14(12):2246-2254. doi:10.7150/jca.84943. https://www.jcancer.org/v14p2246.htm

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Abstract

Background: Prostate cancer is the most common malignant tumor in men, accounting for one of the top five cancer incidences worldwide. However, there is no effective pharmacological treatment for advanced prostate cancer (APC). Herein, we aim to investigate the mechanism of Zhoushi Qiling decoction (ZQD), a traditional Chinese medicine compound, in inhibiting prostate cancer cells proliferation and tumor growth.

Methods: IC50 was determined in PC3 and DU145 cells. Cell viability was determined using MTT assay after interleukin (IL) 6 stimulation. Cell proliferation ability was evaluated using colony formation assay. IL-6/signal transducer and activator of transcription 3 (STAT3) signaling pathway was analyzed using qRT-PCR and Western blot in PC3 and DU145 cells and xenograft tumor tissues.

Results: It was found that ZQD significantly inhibited Il-6-induced cell viability and proliferation in PC3 and DU145 cells. Moreover, ZQD significantly reduced mRNA levels of IL-6, IL-1β, STAT3, Bcl2, and CyclinD1, stimulated by IL-6. The protein levels of p-STAT3, Bcl2 and CyclinD1 were reduced by ZQD treatment at 40 mg/mL both in PC3 and DU145 cells. Additionally, in xenograft tumor tissues, tumor volume, weight and proliferation were significantly reduced by ZQD treatment. In addition, the mRNA and protein levels of IL-6 and pSTAT3 were significantly inhibited by ZQD treatment in vivo.

Conclusion: We demonstrate that ZQD can effectively reduce cell proliferation and tumor growth by inhibiting the activation of IL-6/STAT3 signaling pathway.

Keywords: prostate cancer, Zhoushi Qiling decoction, proliferation, IL-6/STAT3

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APA

Cao, H., Feng, Y., Sun, P., Chen, L., Wang, D., Gao, R. (2023). Zhoushi Qiling decoction inhibits proliferation of human prostate cancer cells through IL6/STAT3 pathway. Journal of Cancer, 14(12), 2246-2254. https://doi.org/10.7150/jca.84943.

ACS

Cao, H.; Feng, Y.; Sun, P.; Chen, L.; Wang, D.; Gao, R. Zhoushi Qiling decoction inhibits proliferation of human prostate cancer cells through IL6/STAT3 pathway. J. Cancer 2023, 14 (12), 2246-2254. DOI: 10.7150/jca.84943.

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CSE

Cao H, Feng Y, Sun P, Chen L, Wang D, Gao R. 2023. Zhoushi Qiling decoction inhibits proliferation of human prostate cancer cells through IL6/STAT3 pathway. J Cancer. 14(12):2246-2254.

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