J Cancer 2019; 10(11):2472-2479. doi:10.7150/jca.29426

Research Paper

MiR-199a-5p suppresses non-small cell lung cancer via targeting MAP3K11

Yanli Li1,*, Detao Wang1,*, Xue Li1,*, Yang Shao1, Yanyun He3, Huansha Yu2,✉, Zhongliang Ma1,✉

1. Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai 200444, China
2. Experimental Animal Center, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China
3. Exprimental Center for Life Sciences, School of Life Sciences, Shanghai University, Shanghai 200444, China
*These authors contributed equally to this work

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Citation:
Li Y, Wang D, Li X, Shao Y, He Y, Yu H, Ma Z. MiR-199a-5p suppresses non-small cell lung cancer via targeting MAP3K11. J Cancer 2019; 10(11):2472-2479. doi:10.7150/jca.29426. Available from http://www.jcancer.org/v10p2472.htm

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Abstract

MicroRNAs (miRNAs) comprise a class of short, non-coding RNAs that directly target 3′UTR of mRNA, causing subsequent degradation or suppression of translation. Here, we verified that miR-199a-5p was significantly down-regulated in mouse NSCLC tissues and human patient samples. To further study the function of miR-199a-5p, lentivirus system was adopted to construct stably over-expressing miR-199a-5p A549, SPC-A1 and H1299 cell lines. Then, miR-199a-5p played a tumor suppression role via directly targeting MAP3K11 gene in non-small cell lung cancer (NSCLC). Elevated miR-199a-5p suppressed cell proliferation and arrested cell cycle in G1 phase. We found that MAP3K11 was negatively correlated with miR-199a-5p in NSCLC patient tissues and mouse xenograft tumors. Our results suggest that miR-199a-5p together with its target gene MAP3K11 is a key factor and constitutes a complicated regulation network in NSCLC.

Keywords: miR-199a-5p, MAP3K11, MAPK pathway, NSCLC, tumor suppression