J Cancer 2017; 8(17):3522-3530. doi:10.7150/jca.20227 This issue Cite

Research Paper

Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer

Boke Zhang1*, Manlin Cao2*, Yiqing He1, Yiwen Liu1, Guoliang Zhang1, Cuixia Yang1, Yan Du1, Jing Xu3, Jiajie Hu1, Feng Gao4✉

1. Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, PR China;
2. Department of Rehabilitation Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, PR China;
3. Department of Clinical Laboratory, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, PR China;
4. Department of Molecular Biology and Clinical Laboratory, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, PR China.
* Both are co-first authors

Citation:
Zhang B, Cao M, He Y, Liu Y, Zhang G, Yang C, Du Y, Xu J, Hu J, Gao F. Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer. J Cancer 2017; 8(17):3522-3530. doi:10.7150/jca.20227. https://www.jcancer.org/v08p3522.htm
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Abstract

Background: Breast cancer (BC)-derived hyaluronan (HA) can induce the formation of M2-like tumor-associated macrophages (TAMs) in tumor context. However, little is known about the correlation between circulating M2-like monocytes and plasma HA in BC patients. This study focused on evaluating the relationship between circulating M2-like monocytes and plasma HA, and further appraised the diagnostic value of them in BC.

Methods: The expression of M2-like TAMs and HA was determined in pathological tissues by immunohistochemistry. Flow cytometry was used to detect the levels of circulating CD14+CD204+ M2-like monocytes in 81 BC patients, 45 patients with breast benign diseases, and 46 healthy subjects. The levels of HA, CEA, and CA15-3 were measured in plasma samples using chemiluminescence method.

Results: M2-like TAMs and HA expressions were elevated in BC tissues compared with benign tissues. In correspondence, the frequency of circulating CD14+CD204+ M2-like monocytes and the plasma HA levels were significantly higher in patients with BC than those in control groups. Importantly, there was a positive correlation between circulating M2-like monocytes and the plasma HA (Spearman r = 0.404, p < 0.001). Area under receiver operating characteristic curve (ROC) for the combination of circulating M2-like monocytes and HA was 0.899 (95% CI: 0.853-0.946), which was higher than the panel of CEA and CA15-3.

Conclusions: The frequency of circulating CD14+CD204+ M2-like monocytes was positively correlated to plasma HA levels. The combination of circulating CD14+CD204+ M2-like monocytes and plasma HA could provide considerable diagnostic value in BC.

Keywords: Breast cancer, CD163, CD204, biomarker, diagnosis


Citation styles

APA
Zhang, B., Cao, M., He, Y., Liu, Y., Zhang, G., Yang, C., Du, Y., Xu, J., Hu, J., Gao, F. (2017). Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer. Journal of Cancer, 8(17), 3522-3530. https://doi.org/10.7150/jca.20227.

ACS
Zhang, B.; Cao, M.; He, Y.; Liu, Y.; Zhang, G.; Yang, C.; Du, Y.; Xu, J.; Hu, J.; Gao, F. Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer. J. Cancer 2017, 8 (17), 3522-3530. DOI: 10.7150/jca.20227.

NLM
Zhang B, Cao M, He Y, Liu Y, Zhang G, Yang C, Du Y, Xu J, Hu J, Gao F. Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer. J Cancer 2017; 8(17):3522-3530. doi:10.7150/jca.20227. https://www.jcancer.org/v08p3522.htm

CSE
Zhang B, Cao M, He Y, Liu Y, Zhang G, Yang C, Du Y, Xu J, Hu J, Gao F. 2017. Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer. J Cancer. 8(17):3522-3530.

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