J Cancer 2012; 3:93-95. doi:10.7150/jca.4163

Show Research Communication

A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel

Yuan Sun^1,2, Kannappan Sriramajayam¹, Dianzhong Luo^2✉, D. Joshua Liao^1✉

1. Hormel Institute, the University of Minnesota, Austin, MN 55912, USA
2. Department of Pathology, Guangxi Medical University, Nanning, Guangxi 530021, P. R. China

Abstract

In this short communication we report a quick, cost-free method of purification of DNA fragments from agarose gel. Unlike those procedures that involve commercial kits, this method uses glass wool or absorbent cotton to filter agarose gel during a quick spinning-down of DNA, thus significantly simplifying the routine practice of many molecular biologists and decreasing the cost.

Keywords: DNA fragments, agarose gel, method of purification.