J Cancer 2018; 9(21):3962-3970. doi:10.7150/jca.25909

Research Paper

Huaier Augmented the Chemotherapeutic Sensitivity of Oxaliplatin via Downregulation of YAP in Hepatocellular Carcinoma

Yuquan Tao1, Liang Shan1, Xin Xu1, Hongyuan Jiang1, Rui Chen1, Zijun Qian1, Zongguo Yang1,2, Bin Liang3, Haiyin Zheng3, Feng Cai3, Yongchun Yu1,4✉, Lifang Ma3✉

1. Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200071, P.R. China.
2. Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, P.R. China.
3. Department of Clinical Laboratory Medicine, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200071, P.R. China.
4. Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, P.R. China.

Abstract

For unresectable Hepatocellular carcinoma (HCC), chemotherapy is still an important treatment strategy. Oxaliplatin (Oxa) is an effective treatment of HCC after sorafenib treatment failure. However, the intrinsic or acquired resistance of Oxa affected the chemotherapeutic sensitivity. By analyzing the data of GEO Database, we found that Oxa aberrantly increased the expression of Cysteine-rich61 (Cyr61) in HCC cell lines. Subsequently, in Bel-7404 and SMMC-7721 cells after treated with Oxa, it was verified that the expression of Cyr61 and Yes-associated protein (YAP) was increased. Moreover, we found that blockade of YAP promoted Oxa-induced cell apoptosis for the first time. Meanwhile, our previous study demonstrated that Huaier (HE) inhibited the expression of YAP. Further study found that combination treatment of Oxa and HE had a significantly synergistic anti-cancer effect and significantly inhibited the expression of YAP and apoptosis related proteins. Taken together, we have observed that overexpression of YAP significantly reduced the chemotherapeutic sensitivity of Oxa in HCC for the first time. Combination treatment of Oxa and HE solved this problem.

Keywords: YAP, chemotherapeutic sensitivity, oxaliplatin, Huaier, synergistic effect, Hepatocellular carcinoma

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How to cite this article:
Tao Y, Shan L, Xu X, Jiang H, Chen R, Qian Z, Yang Z, Liang B, Zheng H, Cai F, Yu Y, Ma L. Huaier Augmented the Chemotherapeutic Sensitivity of Oxaliplatin via Downregulation of YAP in Hepatocellular Carcinoma. J Cancer 2018; 9(21):3962-3970. doi:10.7150/jca.25909. Available from http://www.jcancer.org/v09p3962.htm