J Cancer 2018; 9(21):3867-3873. doi:10.7150/jca.28588
microRNA biomarkers in colorectal cancer liver metastasis
1. Department of Medicine (Division of Genomic Medicine), The George Washington University School of Medicine and Health Sciences, Washington, DC
2. Department of Breast and Thyroid Surgery, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, Hunan, China
3. Department of Colorectal and Anal Surgery, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, Hunan, China
4. Hepatobiliary and enteric Surgery Research Center/Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China
5. Department of Surgery, The George Washington University School of Medicine and Health Sciences, Washington, DC
Huang S, Tan X, Huang Z, Chen Z, Lin P, Fu SW. microRNA biomarkers in colorectal cancer liver metastasis. J Cancer 2018; 9(21):3867-3873. doi:10.7150/jca.28588. Available from http://www.jcancer.org/v09p3867.htm
Liver metastasis is a primary factor of prognosis and long-term survival for patients diagnosed with colorectal cancer (CRC). Colorectal cancer liver metastasis (CRCLM), is a complex biological process involving multiple factors and steps, and its mechanisms are yet to be discovered. In recent years, small noncoding RNAs, especially microRNAs (miRNAs) have been proven to play an important role in tumorigenesis, progression and metastasis in a variety of cancers, including CRC. Increasing evidence suggests that miRNAs, including those from exosomes secreted by tumor cells in circulation could be used as promising biomarkers in early cancer detection, treatment, and prognosis. In this review, we focus on the functional roles and clinical applications of miRNAs, especially those from circulating exosomes secreted by tumor cells related to CRCLM.
Keywords: Colorectal cancer (CRC), liver metastasis, miRNAs, exosome
Colorectal cancer (CRC) is one of the most commonly diagnosed cancers worldwide [1, 2]. CRC is typically treated by surgical resection combined with modern chemo- and radiation- therapies, with a cure rate of ~20% . About 70-80% of CRC patients will develop recurrence and/or metastasis, and more than 50% of the metastatic sites are in the liver [3, 4]. Colorectal cancer liver metastasis (CRCLM) is the leading cause of deaths for CRC . Therefore one of the main research focuses is on elucidating the mechanisms of CRCLM, and identifying potential biomarkers for both prediction and management of CRCLM . Better understanding the biological process of CRCLM will facilitate the development of early diagnostic and therapeutic methods. In recent years, small non-coding RNAs (sncRNAs) have been shown to play a key role in the regulation of gene expression, and are involved in tumor initiation and development. These sncRNAs include small interfering RNAs (siRNAs), microRNAs (miRNAs), PIWI-interacting RNAs (piRNAs), etc., of which miRNAs are studied most extensively and in-depth [7, 8]. In this review, we will focus on the roles and clinical applications of miRNAs in CRCLM, including in circulating exosomes secreted by tumor cells.
miRNAs are endogenous non-coding RNAs of 20-25 nucleotides that regulate gene expression at the post-transcriptional level, through interaction with 3'-untranslated regions (3'UTRs) of the target messenger RNAs (mRNAs) . Each miRNA may target a number of mRNAs, and each mRNA can be regulated by multiple miRNAs. Studies have shown that miRNAs play an important regulatory role in all aspects of CRCLM, including angiogenesis, tumor invasion, epithelial-to-mesenchymal transition (EMT), and stemness of cancer cells . Recently miRNAs have been used in the diagnosis and prognosis of cancer and other diseases . However, metastasis of cancers, such as CRC, is not only challenging to predict, but also has limited and ineffective treatment options. Therefore, it is crucial to discover and validate novel biomarkers that can predict CRCLM in order to improve the survival rate of patients. A number of publications demonstrated that miRNAs, either oncogenic miRNAs (OncomiRs) or tumor suppressor miRNAs, are involved in CRCLM (Table 1).
A list of miRNAs and their validated targets involving CRCLM
|miRNAs||Validated targets||Functional pathways||References|
|miR-155||TP53INP1||Lymph node metastasis|||
|miR-200c||Bmi1, ZEB1, ZEB2||ERK1/2MAPK/GSK-2β/β-catenin signaling, fas signaling|||
|miR-199a-30||HIF-1α, VEGF||Proliferation, motility, invasion|||
|miR-15b||MTSS1, Klotho||Invasion, metastasis|||
A list of miRNAs with their respective clinical applications in CRCLM
|miRNAs||sample||Source of sample||Clinical application/biological function||References|
|miR-29||Tissue mouse model)||CRC/CRCLM||Therapeutic target|||
|miR-622||Tissue/cell lines||CRC/CRCLM||Therapeutic target|||
|miR-21||Cell lines/CAM module||CRC/CRCLM||Therapeutic target|||
|miR-655-3p||Tissue (mouse model)||CRC/CRCLM||Therapeutic target|||
|miR-424-3p,503,1292 (combine together)||Tissue||CRC/CRCLM||Therapeutic target|||
|Exo-miR-19a, 19b, 23a, 92a, 320a, 4437,19a||Serum||CRC/CRCLM||Predict liver metastasis|||
|Exo-miR-7,181a-5p,192,194,375||Serum/cell culture medium||CRC/CRCLM||Inflammation, fibrosis, angiogenesis|||
|Exo-miR-193a||Tissue (mouse model)||CRC/CRCLM||Migration|||
Oncogenic miRNAs (OncomiRs) in CRCLM
OncomiRs, which act like oncogenes by downregulating tumor suppressors and other regulatory genes, have been found to be either amplified or overexpressed in many types of cancers, including CRC. Cellura et al reported that miR-19, significantly overexpressed in CRC cells, acted as an oncomiR, leading to altered invasive ability by inhibiting the expression of Transglutaminase-2 (TG2) which is an important player in colorectal cancer metastasis. TG2 is a critical crosslinking enzyme in the extracellular matrix (ECM) and tumor microenvironment (TME) .Another study showed that up-regulation of miR-885-5p had a significant impact on EMT and overexpression of miR-885-5p significantly induced cell migration, invasion and liver metastasis by targeting cpeb2, which is required for cell cycle progression . miR-20a-5p promotes invasion and metastasis by suppressing Smad4 in CRCLM , while miRNA-21 and miRNA-155 were shown to be overexpressed in many malignant solid tumors, including CRC, thoracic tumors and lung cancer . Shibuya et al found that expression of miR-21 and miR-155 is much higher in tumor tissue compared to the adjacent normal tissue in 156 CRC patients, further demonstrating the correlation between miRNA-21 and miRNA-155, with their respective target genes, PDCD4 and TP53INP1. Interestingly highly expressed miRNA-21 is positively correlated with CRCLM, likely due to the fact that miRNA-21 promotes tumor cell infiltration and metastasis by inhibiting PDCD4, while miR-155 is associated with distant metastasis of lymph nodes. Ji et al  demonstrated that miR-181a promotes tumorigenesis and EMT by inhibiting WIF-1 in CRCLM. miR-429, as an oncomiR, was shown to inhibit apoptosis and induce EMT in CRC by targeting SOX2 . Overexpression of oncomiRs in CRC may promote liver metastasis; therefore, targeting oncomiRs may open a new avenue to prevent CRCLM and improve survival rate of CRC patients.
Tumor suppressor miRNAs in CRCLM
Tumor suppressor miRNAs function as by targeting genes with oncogenic activity. Inactivation or downregulation of tumor suppressor miRNAs was widely reported in many types of cancers including CRC. Bmi1 plays an essential role in Fas-signaling induced stem cell properties in CRC specimens. It was shown that Fas ligand treatment promoted Bmi1 expression by inhibiting miR-200c, a tumor suppressor miRNA . Li et al  discovered that forced expression of miR-26a and miR-26b not only modulates migratory behavior of CRC cells but also inhibits FUT4 expression in CRC cells, resulting in tumor progression. Laudato et al  found that miR-30e-5p inhibits CRCLM by targeting both ITG6 and TGF-B1 directly. Another study showed that there are 939 human miRNAs related to CRCLM. Among them, miR-125, miR-127, miR-145, miR-194 and miR-199a-30, as tumor suppressors, could serve as biomarkers for early diagnosis of CRCLM . Two other recent studies demonstrated that miR-15b inhibits CRCLM by regulating MTSS1 and Klotho , while miRNA-30a-5p (miR-30a) regulates cell motility and EMT by directly targeting oncogenic protein TM4SF1 in CRCLM . Downregulation of tumor suppressor miRNAs may lead to CRCLM and detection of these miRNAs may help manage the progression of CRCLM.
miRNA biomarkers CRCLM
It is crucial to identify and validate novel biomarkers that can predict CRCLM in order to improve patients' survival. Many miRNAs are associated with early diagnosis and staging of CRCLM as we discussed. Therefore, miRNAs can be used as biomarkers for early CRCLM detection , particularly circulating miRNAs for their minimum invasiveness and easy accessibility. One study showed that miR-29a is significantly elevated in serum of CRCLM patients compared to those without metastasis . In fact, miR-29a can discriminate CRCLM patients from non-metastatic CRC patients with 75% of both sensitivity and specificity, which is more sensitive than serum CEA, with only 60% of sensitivity and 34% of specificity. Therefore, serum miR-29a could be a biomarker for CRCLM early detection. In a study of 224 serum samples, Yin et al evaluated the expression of 11 metastasis-associated miRNAs in 116 localized CRC, 72 synchronous liver-metastatic CRC and 36 other organ-metastatic CRC. They found that the expression of serum miR-126, miR-141 and miR-21 in liver metastatic CRC were significantly different from that in localized CRC. Therefore these three miRNAs may be used as novel biomarkers for the clinical diagnosis or prediction of early stage CRCLM. With the identification and validation of more circulating miRNA markers, early detection methods using miRNAs / miRNA signatures may be developed for clinical use in CRCLM.
Therapeutic potentials of miRNAs
It is a new strategy to treat CRCLM by targeting miRNAs, or in combination with other well-established strategies. Clinically, high expression of miR-29 may be predictive for the development of CRCLM. APOBEC3G, involved in CRCLM in a mouse model, was modulated by miR-29-mediated suppression of matrix metalloproteinase-2 (MMP2) to promote CRCLM . Therefore miR-29 may serve not only as a predictive marker for CRCLM, but could also be a therapeutic target. miR-622, which is overexpressed in CRC tissues and cell lines, affects metastasis and invasion of CRC cells by regulating DYRK2 and its downstream signaling pathways. Nedaeinia et al  transfected LS174T human colorectal adenocarcinoma cells with locked nucleic acid (LNA)-anti-miR-21 in vitro to determine the effect of miR-21 in cell growth and colony formation. At the same time, they constructed the LS174T cell chick embryo chorioallantoic membrane (CAM) module. The results showed that the LNA-anti-miR-21 in the CAM module inhibit the expression of colorectal cancer LS174T cells by regulating PDCD4 gene, reducing cell migration and liver metastasis. This provides new ideas for the development of novel miRNA therapeutics and a new direction for the treatment of CRCLM. One recent study  showed that an oligometastatic miR-655-3p was successfully delivered to metastasized liver using nanoscale coordination polymers (NCPs) for a targeted and prolonged distribution of miRNAs. miRNA-655-3p suppressed the tumor growth when co-delivered with oxaliplatin, suggesting additive or synergistic interactions between miRNA and platinum drugs. This was the first time the researchers systemically administered nanoparticles to deliver an oligometastatic miRNA into advanced metastatic hepatic tumors and demonstrated their tumor-suppressive effects. Their findings suggest a potential therapeutic strategy that combines tumor-suppressive miRNAs with conventional cytotoxic chemotherapies for the treatment of CRCLM. However, a single miRNA for CRCLM therapeutic target may not be effective as expected. According to Torres et al , there are 38 miRNAs that are differentially expressed between highly metastatic (KM12SM/SW620) and poorly metastatic (KM12C/SW480) CRC cell lines. They found that three miRNAs (miR-424-3p, miR-503 and miR-1292) were overexpressed in both metastatic CRC cell lines and patient samples. Down-regulation of these three miRNAs shared same targets, CKB and UBA2, which increased cell adhesion and proliferation in CRC cells. This three-miRNA panel along with their two targets may constitute therapeutic biomarkers for CRCLM, suggesting that targeting multiple miRNAs may be a more efficient therapeutic strategy.
Prognostic values of miRNAs
It is suggested that miRNAs can not only assess the risks of tumor metastasis and recurrence, but also evaluate the responses of therapeutics and predict the chemo-resistance to specific drugs in CRCLM. Hur et al  experimentally verified a number of miRNAs specifically associated with CRCLM in tissue and serum, which could be used to determine the prognosis. They found twenty-three miRNAs that are aberrantly expressed by comparing CRC with- and without- metastasis. Among them, four miRNAs (let-7i, miR-10b, miR-221 and miR-320a) were down-regulated in the liver metastasis group, and one (miR-885-5p) was up-regulated in the non-metastatic CRC group. It is suggested that low let-7i expression predicts possible distant metastasis and worse prognosis, whereas high miR-10b expression indicates a high incidence of distant metastasis . High expression of miRNA-885-5p in serum is associated with lymph node and distant metastasis. The results were subsequently validated by RNA in situ nucleic acid hybridization. A similar study  revealed that miR-214 was downregulated in CRCLM, which is associated with the increased expression of FGFR-1, a target of miR-214. The data suggest that miR-214 could be used as a biomarker for poor prognosis of CRCLM patients. Lou et al  found that miR-625 was significantly down-regulated in CRC tissues and cell lines, which is associated with lymph node metastasis and liver metastasis. Both in vitro and in vivo experiments demonstrated that the expression of ectopic miR-625 inhibited the invasion and metastasis of HCT116 CRC cell line. At the same time, low miR-625 expression is associated with low survival rate, indicating that miR-625 can be used as an indicator of poor prognosis of CRC patients. miRNA-122  was reported to be differentially expressed in CRCLM clinical specimens by laser capture microdissection (LCM). As a target of miR-122, cationic amino acid transporter 1 (CAT1) is expressed significantly lower in CRCLM compared to that in CRC. The analysis of 132 primary tumors revealed that the expression level of CAT1 was negatively correlated with liver metastases, and the overexpression of miRNA-122 was associated with downregulated CAT1 expression. Taken together, these data suggest that CAT1 and miR-122 could predict the prognosis of liver metastasis in CRC patients. According to some other recent studies, miR-10b , miR-196b-5p and miR-203[40, 41] are independent risk factors for overall survival in CRC patients with liver metastases. Despite the progress being made, there is much more research work to be done in both animal models and clinical trials to uncover the full potential of miRNA biomarkers in CRCLM.
Circulating exosomal miRNAs in CRCLM
Exosomes are associated with cancer progression and can act in a paracrine or endocrine manner to affect cell behaviors . With the progression of exosome studies in recent years, especially the discovery of the intercellular communications through exosomes and other extracellular vesicles, the potential clinical application of miRNAs and/or proteins from secreted exosomes has generated a lot of interest in the cancer research field. An exosome is a type of lipid nanoparticle with a diameter of 30-100 nm that are released and secreted by various living cells, containing internal proteins, mRNAs, miRNAs and other substances . They can be transmitted in the circulation system and eventually absorbed by other cells. Cancer-secreted exosomes influence the tumor microenvironment and affects the growth and metastasis of cancer cells. Exosomes are a newly discovered communication medium for intercellular transporters and nucleic acid substances. They are involved in a variety of cellular processes, such as intercellular communication, cell migration, angiogenesis and immune regulation. Compared to direct cell contact or secretory factors, exosomes are more targeted and can affect the function of recipient cells more efficiently. They play an important role in cancer, infectious diseases and neurodegenerative diseases. Currently, there are no standard agency-approved, exosome-based diagnostic, therapeutic, prognostic clinical tests for CRCLM. miRNAs contained in exosomes derived from CRC cells are closely related to oncogenes and tumor metastasis-related genes, suggesting that they may be related to CRCLM, and play a role in the initiation and development of CRCLM .
Compared to healthy population, cancer patients have larger numbers of circulating exosomes . Therefore, circulating exosomal miRNAs may be used for diagnosis or prognosis for CRC patients. Reliable blood test could be a perfect companion tool for routine imaging examination of CRCLM patients or for early detection of CRCLM. Exosomal miRNAs are considered the main source of circulating miRNAs isolated from serum or plasma . Currently, there are a few studies showing the analysis of circulating exosomal miRNAs in early diagnosis of CRCLM. Matsumura et al  analyzed the expression of exosomal miRNAs in 227 serum samples from CRC patients. Compared with patients without recurrence, expression of 18 exosomal miRNAs was increased and 46 decreased. Among them, six exosomal miRNAs (miR-19a, miR-19b, miR-23a, miR-92a, miR-320a and miR-4437) were associated with the development of liver metastasis, rendering them to be potential biomarkers for CRCLM early detection. Further studies suggested that the CRC patients with high level of exosomal miR-19a expression were associated with poor prognosis compared to the low expression group. This may indicate that the exosomal miR-19a could be a potential marker of prognosis in CRCLM.
At present, there are a few studies that focus on the clinical applications of circulating exosomal miRNAs in CRCLM. The stability and reproducibility of exosomal miRNA detection and its independent features require more research to evaluate and validate. But the potential clinical impact on CRCLM cannot be ignored. As the research technology matures and deepens, the detection of circulating exosomal miRNAs may become a very promising field for finding CRCLM biomarkers.
In recent studies, the functional studies in cancer cell lines or xenograft models have revealed the mechanisms of exosomal miRNAs in cancer cells or another cell type of TME in supporting or inhibiting tumor growth, transferring drug resistance, and preparing for the metastatic niche .
Based on the study by Fu et al , there are 25 miRNAs being up-regulated and 5 miRNAs being down-regulated in exosomes purified from SW620 culture supernatant. Candidate miRNAs were further evaluated for CRC diagnosis by using qRT-PCR. High expression levels of circulating exosomal miR-17-5p and miR-92a-3p were associated with the stages of CRC patients, indicating that these exosomal miRNAs could be biomarkers for CRC and / or CRCLM. The data further showed that exosomal miR-7, miR-181a-5p, miR-192, miR-194, and miR-375, which are related to multiple biofunctions such as inflammation, fibrosis, and angiogenesis, may also play an important role in CRCLM.
Valcz et al  detected the expression of the Alix protein, which are stably present in exosomes, from normal intestinal epithelial tissue, adenoma tissue and colorectal cancer tissue. They discovered that exosomes play an important role in promoting the carcinogenesis of colonic epithelium and migration. Recent studies show that the expression of exosomal miR-210 was significantly higher compared with its intracellular levels in adherently growing HCT-8 cells, and correlated to anoikis resistance and EMT markers. Exosomes can induce cancerous growth of normal epithelial cells by promoting EMT through miRNA-210  and can affect the adhesion and migration of CRC cells.
Teng et al  examined different exosomal miRNA expression profiles in primary mouse colon tumor, CRCLM and naïve colon tissues. They found that exosomal miR-193a interacts with major vault protein (MVP), causing cell cycle G1 arrest and cell proliferation repression by targeting Caprin1. This suggests that MVP-mediated selective sorting of tumor suppressor miRNA into exosomes promotes tumor progression and liver migration.
We are still in the early stages of exosomal research in CRCLM. Since exosomes and exosomal miRNAs can be detected from patients non-invasively through blood, urine or other body fluids, these microvesicles could be potential sources of diagnostic, prognostic or predictive biomarkers of CRCLM. It is likely that the studies focusing on circulating exosomal miRNAs analysis in patient blood, urine or other body as liquid biopsy research are a step forward towards precision medicine.
Discussion and conclusions
Liver metastasis is an important biological feature of CRC, and it is also an important factor of recurrence and poor prognosis of CRC patients. Combining molecular and traditional treatments may lead to an improved Overall Survival (OS), a reduction in intrahepatic recurrence, and a decreased toxicity of perioperative therapies. The process of liver metastasis of CRC depends on multiple complex interactions between cancer cells in the tumor and host-derived cells in the microenvironment in both the primary tumor and secondary organs. In contrast to the complex and diverse oncogenes and tumor suppressor genes involved in cancer, miRNAs with tumor promoting or tumor suppressing effects have many more advantages . Identification of miRNAs, including exosomal miRNAs, involving in liver metastasis could lead to the development of more accurate detection methods for early diagnosis, treatment and prognosis of CRCLM. With the continued improvements of miRNA expression profiling, and the development of more stable, effective and low-toxic miRNA mimics and inhibitors, the number of drugs targeting miRNAs has increased at much faster pace. Although the research and the analysis on miRNAs especially the exosomal miRNAs in CRCLM are full of challenges, the prospects are still very broad. The role of miRNAs in CRCLM will receive more extensive attention. It seems that miRNA-based therapeutics as adjuvant tools of targets will become realities in the near future after the technical limitations are overcome.
This research was supported by the Elaine H. Snyder Cancer Research Award (to SWF).
Conflicts of Interest
The authors declare that they have no competing interests.
1. Strubberg AM, Madison BB. MicroRNAs in the etiology of colorectal cancer: pathways and clinical implications. Dis Model Mech. 2017;10(3):197-214
2. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018. CA Cancer J Clin. 2018;68(1):7-30
3. Tomlinson JS, Jarnagin WR, DeMatteo RP, Fong Y, Kornprat P, Gonen M, Kemeny N, Brennan MF, Blumgart LH, D'Angelica M. Actual 10-year survival after resection of colorectal liver metastases defines cure. J Clin Oncol. 2007;25(29):4575-4580
4. House MG, Kemeny NE, Gonen M, Fong Y, Allen PJ, Paty PB, DeMatteo RP, Blumgart LH, Jarnagin WR, D'Angelica MI. Comparison of adjuvant systemic chemotherapy with or without hepatic arterial infusional chemotherapy after hepatic resection for metastatic colorectal cancer. Ann Surg. 2011;254(6):851-856
5. Misiakos EP, Karidis NP, Kouraklis G. Current treatment for colorectal liver metastases. World J Gastroenterol. 2011;17(36):4067-4075
6. Zarour LR, Anand S, Billingsley KG, Bisson WH, Cercek A, Clarke MF, Coussens LM, Gast CE, Geltzeiler CB, Hansen L. et al. Colorectal Cancer Liver Metastasis: Evolving Paradigms and Future Directions. Cell Mol Gastroenterol Hepatol. 2017;3(2):163-173
7. Schetter AJ, Okayama H, Harris CC. The role of microRNAs in colorectal cancer. Cancer J. 2012;18(3):244-252
8. Wang D, Liu J, Huo T, Tian Y, Zhao L. The role of microRNAs in colorectal liver metastasis: Important participants and potential clinical significances. Tumour Biol. 2017;39(6):1010428317709640
9. Bracken CP, Scott HS, Goodall GJ. A network-biology perspective of microRNA function and dysfunction in cancer. Nat Rev Genet. 2016;17(12):719-732
10. Lin M, Chen W, Huang J, Gao H, Ye Y, Song Z, Shen X. MicroRNA expression profiles in human colorectal cancers with liver metastases. Oncol Rep. 2011;25(3):739-747
11. Peng Y, Croce CM. The role of MicroRNAs in human cancer. Signal Transduct Target Ther. 2016;1:15004
12. Cellura D, Pickard K, Quaratino S, Parker H, Strefford JC, Thomas GJ, Mitter R, Mirnezami AH, Peake NJ. miR-19-Mediated Inhibition of Transglutaminase-2 Leads to Enhanced Invasion and Metastasis in Colorectal Cancer. Mol Cancer Res. 2015;13(7):1095-1105
13. Lam CSC, Ng L, Chow AKM, Wan TMH, Yau S, Cheng NSM, Wong SKM, Man JHW, Lo OSH, Foo DCC. et al. Identification of microRNA 885-5p as a novel regulator of tumor metastasis by targeting CPEB2 in colorectal cancer. Oncotarget. 2017;8(16):26858-26870
14. Cheng D, Zhao S, Tang H, Zhang D, Sun H, Yu F, Jiang W, Yue B, Wang J, Zhang M. et al. MicroRNA-20a-5p promotes colorectal cancer invasion and metastasis by downregulating Smad4. Oncotarget. 2016;7(29):45199-45213
15. Yan LX, Huang XF, Shao Q, Huang MY, Deng L, Wu QL, Zeng YX, Shao JY. MicroRNA miR-21 overexpression in human breast cancer is associated with advanced clinical stage, lymph node metastasis and patient poor prognosis. Rna. 2008;14(11):2348-2360
16. Shibuya H, Iinuma H, Shimada R, Horiuchi A, Watanabe T. Clinicopathological and prognostic value of microRNA-21 and microRNA-155 in colorectal cancer. Oncology. 2010;79(3-4):313-320
17. Ji D, Chen Z, Li M, Zhan T, Yao Y, Zhang Z, Xi J, Yan L, Gu J. MicroRNA-181a promotes tumor growth and liver metastasis in colorectal cancer by targeting the tumor suppressor WIF-1. Mol Cancer. 2014;13:86
18. Li J, Du L, Yang Y, Wang C, Liu H, Wang L, Zhang X, Li W, Zheng G, Dong Z. MiR-429 is an independent prognostic factor in colorectal cancer and exerts its anti-apoptotic function by targeting SOX2. Cancer Lett. 2013;329(1):84-90
19. Chen J, Wang Y, Zhuo L, Liu Z, Liu T, Li W, Cai Y, Zheng H. Fas signaling induces stemness properties in colorectal cancer by regulation of Bmi1. Mol Carcinog. 2017;56(10):2267-2278
20. Li Y, Sun Z, Liu B, Shan Y, Zhao L, Jia L. Tumor-suppressive miR-26a and miR-26b inhibit cell aggressiveness by regulating FUT4 in colorectal cancer. Cell Death Dis. 2017;8(6):e2892
21. Laudato S, Patil N, Abba ML, Leupold JH, Benner A, Gaiser T, Marx A, Allgayer H. P53-induced miR-30e-5p inhibits colorectal cancer invasion and metastasis by targeting ITGA6 and ITGB1. Int J Cancer. 2017;141(9):1879-1890
22. Pecqueux M, Liebetrau I, Werft W, Dienemann H, Muley T, Pfannschmidt J, Mussle B, Rahbari N, Scholch S, Buchler MW. et al. A Comprehensive MicroRNA Expression Profile of Liver and Lung Metastases of Colorectal Cancer with Their Corresponding Host Tissue and Its Prognostic Impact on Survival. Int J Mol Sci. 2016:17 (10)
23. Li J, Chen Y, Guo X, Zhou L, Jia Z, Tang Y, Lin L, Liu W, Ren C. Inhibition of miR-15b decreases cell migration and metastasis in colorectal cancer. Tumour Biol. 2016;37(7):8765-8773
24. Park YR, Kim SL, Lee MR, Seo SY, Lee JH, Kim SH, Kim IH, Lee SO, Lee ST, Kim SW. MicroRNA-30a-5p (miR-30a) regulates cell motility and EMT by directly targeting oncogenic TM4SF1 in colorectal cancer. J Cancer Res Clin Oncol. 2017;143(10):1915-1927
25. Manne U, Jadhav T, Putcha BK, Samuel T, Soni S, Shanmugam C, Suswam EA. Molecular Biomarkers of Colorectal Cancer and Cancer Disparities: Current Status and Perspective. Curr Colorectal Cancer Rep. 2016;12(6):332-344
26. Wang LG, Gu J. Serum microRNA-29a is a promising novel marker for early detection of colorectal liver metastasis. Cancer Epidemiol. 2012;36(1):e61-67
27. Yin J, Bai Z, Song J, Yang Y, Wang J, Han W, Zhang J, Meng H, Ma X, Yang Y. et al. Differential expression of serum miR-126, miR-141 and miR-21 as novel biomarkers for early detection of liver metastasis in colorectal cancer. Chin J Cancer Res. 2014;26(1):95-103
28. Ding Q, Chang CJ, Xie X, Xia W, Yang JY, Wang SC, Wang Y, Xia J, Chen L, Cai C. et al. APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis. J Clin Invest. 2011;121(11):4526-4536
29. Wang Y, Sun J, Wei X, Luan L, Zeng X, Wang C, Zhao W. Decrease of miR-622 expression suppresses migration and invasion by targeting regulation of DYRK2 in colorectal cancer cells. Onco Targets Ther. 2017;10:1091-1100
30. Nedaeinia R, Sharifi M, Avan A, Kazemi M, Nabinejad A, Ferns GA, Ghayour-Mobarhan M, Salehi R. Inhibition of microRNA-21 via locked nucleic acid-anti-miR suppressed metastatic features of colorectal cancer cells through modulation of programmed cell death 4. Tumour Biol. 2017;39(3):1010428317692261
31. Oshima G, Guo N, He C, Stack ME, Poon C, Uppal A, Wightman SC, Parekh A, Skowron KB, Posner MC. et al. In Vivo Delivery and Therapeutic Effects of a MicroRNA on Colorectal Liver Metastases. Mol Ther. 2017;25(7):1588-1595
32. Torres S, Garcia-Palmero I, Bartolome RA, Fernandez-Acenero MJ, Molina E, Calvino E, Segura MF, Casal JI. Combined miRNA profiling and proteomics demonstrates that different miRNAs target a common set of proteins to promote colorectal cancer metastasis. J Pathol. 2017;242(1):39-51
33. Hur K, Toiyama Y, Schetter AJ, Okugawa Y, Harris CC, Boland CR, Goel A. Identification of a metastasis-specific MicroRNA signature in human colorectal cancer. J Natl Cancer Inst. 2015:107 (3)
34. Ma L, Teruya-Feldstein J, Weinberg RA. Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature. 2007;449(7163):682-688
35. Chen DL, Wang ZQ, Zeng ZL, Wu WJ, Zhang DS, Luo HY, Wang F, Qiu MZ, Wang DS, Ren C. et al. Identification of microRNA-214 as a negative regulator of colorectal cancer liver metastasis by way of regulation of fibroblast growth factor receptor 1 expression. Hepatology. 2014;60(2):598-609
36. Lou X, Qi X, Zhang Y, Long H, Yang J. Decreased expression of microRNA-625 is associated with tumor metastasis and poor prognosis in patients with colorectal cancer. J Surg Oncol. 2013;108(4):230-235
37. Iino I, Kikuchi H, Miyazaki S, Hiramatsu Y, Ohta M, Kamiya K, Kusama Y, Baba S, Setou M, Konno H. Effect of miR-122 and its target gene cationic amino acid transporter 1 on colorectal liver metastasis. Cancer Sci. 2013;104(5):624-630
38. Nishida N, Yamashita S, Mimori K, Sudo T, Tanaka F, Shibata K, Yamamoto H, Ishii H, Doki Y, Mori M. MicroRNA-10b is a Prognostic Indicator in Colorectal Cancer and Confers Resistance to the Chemotherapeutic Agent 5-Fluorouracil in Colorectal Cancer Cells. Ann Surg Oncol. 2012;19(9):3065-3071
39. Stiegelbauer V, Vychytilova-Faltejskova P, Karbiener M, Pehserl AM, Reicher A, Resel M, Heitzer E, Ivan C, Bullock M, Ling H. et al. miR-196b-5p Regulates Colorectal Cancer Cell Migration and Metastases through Interaction with HOXB7 and GALNT5. Clin Cancer Res. 2017;23(17):5255-5266
40. Li WH, Chang JJ, Tong D, Peng JJ, Huang D, Guo WJ, Zhang W, Li J. Differential microRNA expression profiling in primary tumors and matched liver metastasis of patients with colorectal cancer. Oncotarget. 2017;8(22):35783-35791
41. Hur K, Toiyama Y, Okugawa Y, Ide S, Imaoka H, Boland CR, Goel A. Circulating microRNA-203 predicts prognosis and metastasis in human colorectal cancer. Gut. 2017;66(4):654-665
42. Carvalho J, Oliveira C. Extracellular Vesicles - Powerful Markers of Cancer EVolution. Front Immunol. 2014;5:685
43. Ruiz-Lopez L, Blancas I, Garrido JM, Mut-Salud N, Moya-Jodar M, Osuna A, Rodriguez-Serrano F. The role of exosomes on colorectal cancer: A review. J Gastroenterol Hepatol. 2018;33(4):792-799
44. Matsumura T, Sugimachi K, Iinuma H, Takahashi Y, Kurashige J, Sawada G, Ueda M, Uchi R, Ueo H, Takano Y. et al. Exosomal microRNA in serum is a novel biomarker of recurrence in human colorectal cancer. Br J Cancer. 2015;113(2):275-281
45. Kalluri R. The biology and function of exosomes in cancer. J Clin Invest. 2016;126(4):1208-1215
46. Nedaeinia R, Manian M, Jazayeri MH, Ranjbar M, Salehi R, Sharifi M, Mohaghegh F, Goli M, Jahednia SH, Avan A. et al. Circulating exosomes and exosomal microRNAs as biomarkers in gastrointestinal cancer. Cancer Gene Ther. 2017;24(2):48-56
47. Pencheva N, Tavazoie SF. Control of metastatic progression by microRNA regulatory networks. Nat Cell Biol. 2013;15(6):546-554
48. Fu F, Jiang W, Zhou L, Chen Z. Circulating Exosomal miR-17-5p and miR-92a-3p Predict Pathologic Stage and Grade of Colorectal Cancer. Transl Oncol. 2018;11(2):221-232
49. Valcz G, Galamb O, Krenacs T, Spisak S, Kalmar A, Patai AV, Wichmann B, Dede K, Tulassay Z, Molnar B. Exosomes in colorectal carcinoma formation: ALIX under the magnifying glass. Mod Pathol. 2016;29(8):928-938
50. Bigagli E, Luceri C, Guasti D, Cinci L. Exosomes secreted from human colon cancer cells influence the adhesion of neighboring metastatic cells: Role of microRNA-210. Cancer Biol Ther. 2016:1-8
51. Teng Y, Ren Y, Hu X, Mu J, Samykutty A, Zhuang X, Deng Z, Kumar A, Zhang L, Merchant ML. et al. MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression. Nat Commun. 2017;8:14448
52. Dong Y, Wu WK, Wu CW, Sung JJ, Yu J, Ng SS. MicroRNA dysregulation in colorectal cancer: a clinical perspective. Br J Cancer. 2011;104(6):893-898
Corresponding author: Sidney W. Fu, M.D., Ph.D. Department of Medicine, Division of Genomic Medicine, The George Washington University School of Medicine and Health Sciences, 2300 Eye Street, N.W. Ross Hall 402C, Washington, DC 20037 Email: sfuedu Tel: 202-994-4767